The spliceosome is a large ribonucleoprotein complex that removes introns from pre-
mRNAs. At its functional core lies the essential pre-mRNA processing factor 8 (Prp8) protein …

As the Cne PRP8 intein is active and exists in an essential gene of an important fungal
pathogen, inhibitors of splicing and assays for intein activity are of interest. The self-splicing …

The occurrence of spliceosomal introns in eukaryotic genomes is highly diverse and ranges
from few introns in an organism to multiple introns per gene. Introns vary with respect to their …

Removal of pre-mRNA introns is an essential step in eukaryotic genome interpretation. The
spliceosome, a ribonucleoprotein performs this critical function; however, precise roles for …

We determined that over 40 spliceosomal proteins are conserved between many fungal
species and humans but were lost during the evolution of S. cerevisiae, an intron-poor yeast …

Prp8 is the largest and most highly conserved protein in the spliceosome yet its mechanism
of function is poorly understood. Our previous studies implicate Prp8 in control of …

The genome of the fission yeast Schizosaccharomyces pombe encodes 17 kinases that are
essential for cell growth. These include the cell-cycle regulator Cdc2, as well as several …

Prp8 stands out among hundreds of splicing factors as a protein that is intimately involved in
spliceosomal activation and the catalytic reaction. Here, we present the first comprehensive …

The alternative splicing (AS) of precursor messenger RNA (pre‐mRNA) is a tightly regulated
process through which introns are removed to leave the resulting exons in the mRNA …

In the yeast U1 snRNP the Prp39/Prp42 heterodimer is essential for early steps of
spliceosome assembly. In metazoans no Prp42 ortholog exists, raising the question how the …