The introduction of real-time PCR technology has significantly improved and simplified the
quantification of nucleic acids, and this technology has become an invaluable tool for many …

With a variety of detection chemistries, an increasing number of platforms, multiple choices
for analytical methods and the jargon emerging along with these developments, real-time …

Real-time fluorescence-based quantitative PCR has become established as the benchmark
technology for the quantification of nucleic acids, offering an immense choice of protocols …

Real‐time PCR is a recent modification to the polymerase chain reaction that allows precise
quantification of specific nucleic acids in a complex mixture by fluorescent detection of …

The polymerase chain reaction (PCR) has become one of the most important tools in
molecular diagnostics, providing exquisite sensitivity and specificity for detection of nucleic …

Real‐time PCR is a recent modification to the polymerase chain reaction that allows precise
quantification of specific nucleic acids in a complex mixture by fluorescent detection of …

The repeatability and sensitivity of a simple, adaptable, semi-quantitative, realtime RT-PCR
assay was investigated. The assay can be easily and rapidly applied to quantitate relative …

Real‐time PCR is being used increasingly as the method of choice for mRNA quantification,
allowing rapid analysis of gene expression from low quantities of starting template. Despite …

Background Real-time PCR analysis is a sensitive DNA quantification technique that has
recently gained considerable attention in biotechnology, microbiology and molecular …

The MIQE (minimum information for the publication of quantitative real-time PCR) guidelines
were published in 2009 with the twin aims of providing a blueprint for good real-time …