Expression and purification of recombinant swine interleukin-4

A Nuntaprasert, Y Mori, K Fujita, M Yoneda… - … and infectious diseases, 2005 - Elsevier
The swine interleukin-4 (SwIL-4) cDNA was cloned by RT-PCR. It was expressed using an
expression vector pQE30 in E. coli, a baculovirus AcNPV vector pVL1392 in insect cells, and …

Molecular cloning and characterization of a ruminant interleukin‐6 cDNA

AE Andrews, GJ Barcham, K Ashman… - Immunology and cell …, 1993 - Wiley Online Library
By hybridization with a human interleukin‐6 (IL‐6) cDNA fragment a corresponding ruminant
(ovine) cDNA was isolated from a lipopolysaccharide (LPS)‐stimulated alveolar …

Bovine interleukin 2 (IL-2) production and activity on bovine and murine cell lines

M Miller-Edge, GA Splitter - Veterinary Immunology and Immunopathology, 1984 - Elsevier
Long-term growth of T cell cultures requires addition of Interleukin 2 (IL-2). In order to
maintain bovine cultures, optimal conditions for bovine IL-2 production were defined using …

Characterization of caprine interleukin-4

KR Snekvik, JC Beyer, G Bertoni, BR Von Beust… - Veterinary immunology …, 2001 - Elsevier
Caprine interleukin-4 (IL-4) cDNA was cloned from RNA of mitogen stimulated goat
peripheral blood mononuclear cells utilizing reverse transcriptase–polymerase chain …

Bovine interleukin 2: production and characterization

SR Sambhara, EL Belden - Veterinary Immunology and Immunopathology, 1988 - Elsevier
The production of bovine IL 2 was studied and IL 2 was partially characterized. PMA at 5
ng/ml+ Concanavalin A at 5 μg/ml treatment of peripheral blood mononuclear cells gave a …

Molecular cloning, expression, and purification of pig interleukin-5

H Sylvin, O Matvienko, A Leonchiks, K Alving… - Immunogenetics, 2000 - Springer
Abstract Interleukin-5 (IL-5) is thought to be a key cytokine in allergic inflammation. Pig IL-5
was cloned, sequenced, and expressed to enable us to study of the biological role of IL-5 in …

Development of detection methods for ruminant interleukin (IL)-4

JC Hope, LS Kwong, M Thom, P Sopp… - Journal of …, 2005 - Elsevier
Recombinant bovine IL-4 (rbo IL-4) was transiently expressed in COS-7 cells. Mice were
immunised with a plasmid encoding rbo IL-4 and boosted with rbo IL-4. A number of …

Expression of recombinant porcine interleukin-2 and application of its antibody to immunoassays

IS Choi, HS Yoo - Journal of Veterinary Science, 2002 - synapse.koreamed.org
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response
regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear …

Molecular cloning and mRNA expression of porcine interleukin-12

DL Foss, MP Murtaugh - Veterinary immunology and immunopathology, 1997 - Elsevier
Interleukin (IL)-12 is a heterodimeric cytokine consisting of 35 and 40 kDa subunits,
produced primarily by phagocytic cells in response to bacteria or bacterial products. IL-12 is …

Canine interleukin-5: molecular characterization of the gene and expression of biologically active recombinant protein

S Yang, KS Sellins, E Weber… - Journal of Interferon & …, 2001 - liebertpub.com
Interleukin-5 (IL-5), which is produced primarily by type 2 T helper lymphocytes (Th2), is an
eosinophil differentiation and activation factor. Increased numbers of eosinophils in …