The human monocytic cell line, THP-1, is the most widely used model for primary human
monocytes/macrophages. This is because, following differentiation using phorbol 12 …

Objectives: The differentiation of THP-1 monocytes into macrophages is mainly conducted at
a phorbol 12-myristate 13-acetate (PMA) concentration of 10–400 ng/ml. However, this …

Understanding macrophage biology can improve comprehension of diverse biological
processes and provide insights into novel therapeutic immunomodulatory strategies. Due to …

Human peripheral-blood monocytes are used as an established in vitro system for
generating macrophages. For several reasons, monocytic cell lines such as THP-1 have …

In vitro models of differing macrophage functions are useful since human monocyte–derived
macrophages are short–lived, finite and vary from donor to donor. Published protocols using …

Differentiated macrophages are the resident tissue phagocytes and sentinel cells of the
innate immune response. The phenotype of mature tissue macrophages represents the …

Macrophages are versatile cells of the immune system which react to various external stimuli
through different polarization patterns which adjust the cells to the required function whether …

Macrophages derived from human monocytic leukemia THP-1 cell line are often used as the
alternative of human primary macrophage. However, the polarization method of THP-1 to …

THP-1 is a human leukemia monocytic cell line, which has been extensively used to study
monocyte/macrophage functions, mechanisms, signaling pathways, and nutrient and drug …

Macrophages are key in orchestrating immune responses to micro-environmental stimuli,
sensed by a complex set of surface receptors. The human cell line THP-1 has a monocytic …