The beginning of this century has witnessed great advances in the understanding of ovarian
physiology and embryo development, in the improvement of assisted reproductive …

Precise genome editing of large animals applied to livestock and biomedicine is nowadays
possible since the CRISPR revolution. This review summarizes the latest advances and the …

Contents Genome modification at specific loci in livestock species was only achievable by
performing homologous recombination in somatic cells followed by somatic cell nuclear …

Genetic modification is a rapidly developing field in which numerous significant
breakthroughs have been achieved. Over the last few decades, genetic modification has …

The ablation (KO) or targeted insertion (KI) of specific genes or sequences has been
essential to test their roles on a particular biological process. Unfortunately, such genome …

This article provides an overview of assisted reproductive technologies (ART) and genome
engineering to improve livestock production systems for the contribution of global …

The ability to manipulate the genome by adding, removing, or modifying DNA sequences in
a sequence-specific fashion is essential to studies that investigate the genetic underpinning …

The implementation of CRISPR technology in large animals requires further improvements
in embryo manipulation and transfer to be applied with commercial purposes. In this study …

Humans are sorely over-dependent on livestock for their daily basic need of food in the form
of meat, milk, and eggs. Therefore, genetic engineering and transgenesis provide the …

Transgenic technologies used for creating a desired genomic change in animals involve
three critical steps: isolation of fertilized eggs, microinjection of transgenic DNA into them …