Mouse spermatogonial stem cells (SSCs) can be cultured for multiplication and maintained
for long periods while preserving their spermatogenic ability. Although the cultured SSCs …

Organisms with targeted genomic modifications are efficiently produced by gene editing in
embryos using CRISPR/Cas9 RNA-guided DNA endonuclease. Here, to facilitate germline …

Spermatogonial stem cells (SSCs) can produce numerous male gametes after
transplantation into recipient testes, presenting a valuable approach for gene therapy and …

Precise genome editing is a powerful tool for analysis of gene function. However, in
spermatogonial stem cells (SSCs), this still remains a big challenge mainly due to low …

BACKGROUND Subfertility affects approximately 15% of all couples, and a severe male
factor is identified in 17% of these couples. While the etiology of a severe male factor …

Editing the genome to create specific sequence modifications is a powerful way to study
gene function and promises future applicability to gene therapy. Creation of precise …

Gene expression analyses suggest that more than 1000–2000 genes are expressed
predominantly in mouse and human testes. Although functional analyses of hundreds of …

More than 1000 genes are predicted to be predominantly expressed in mouse testis, yet
many of them remain unstudied in terms of their roles in spermatogenesis and sperm …

Male germ cells undergo complex developmental processes eventually producing
spermatozoa through spermatogenesis, although the molecular mechanisms remain largely …

Conditional knockout (cKO) mice have contributed greatly to understanding the tissue-or
stage-specific functions of genes in vivo. However, the current cKO method requires …