Optical biosensors, including the BIACORE, provide an increasingly popular method for
determining reaction rates of biomolecules. In a flow chamber, with one reactant …

Surface-based binding assays are often influenced by the transport of analyte to the sensor
surface. Using simulated data sets, we test a simple two-compartment model to see if its …

The transport and kinetic processes describing biomolecular interactions in the BIACORE
optical biosensor have been studied with the help of a mathematical model. In comparison …

Accurate estimation of biomolecular reaction rates from binding data, when ligands in
solution bind to receptors on the surfaces of cells or biosensors, requires an understanding …

Many cellular reactions involve a reactant in solution binding to or dissociating from a
reactant confined to a surface. This is true as well for a BIAcore TM, an optical biosensor that …

Many cellular reactions involve a reactant in solution binding to or dissociating from a
reactant attached to a surface. Most studies assume that the reactions occur on this surface …

Rate constants that characterize the kinetics of binding and dissociation between
biomolecules carry fundamental information about the biological processes these molecules …

Optical, evanescent wave biosensors have become popular tools for quantitatively
characterizing the kinetic properties of biomolecular interactions. Analyzing data from …

When estimating rate constants using the BIAcore surface plasmon resonance (SPR)
biosensor, one must have an accurate mathematical model to interpret sensogram data …

Figure 1 Global fit of a two-compartment reaction model (Eqn 1, red lines) to binding
responses generated for the same reaction over two different capacity surfaces6. The …