Polymerase chain reaction (PCR)-based assays can target either DNA (the genome) or RNA
(the transcriptome). Targeting the genome generates robust data that are informative and …

Abstract BACKGROUND Digital PCR (dPCR) is an increasingly popular manifestation of
PCR that offers a number of unique advantages when applied to preclinical research …

Reverse transcription PCR (RT-PCR) represents a sensitive and powerful tool for analyzing
RNA. While it has tremendous potential for quantitative applications, a comprehensive …

The repeatability and sensitivity of a simple, adaptable, semi-quantitative, realtime RT-PCR
assay was investigated. The assay can be easily and rapidly applied to quantitate relative …

Two-step quantitative real-time RT-PCR (RT-qPCR), also known as real-time RT-PCR,
kinetic RT-PCR, or quantitative fluorescent RT-PCR, has become the method of choice for …

The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774
deaths and caused significant economic damage worldwide. Since July 2003, a number of …

Combining multiplex PCR, sequentially, with reverse line blot hybridization (mPCR/RLB) is a
convenient, objective way to identify up to 43 targets in 43 individual specimens …

With a variety of detection chemistries, an increasing number of platforms, multiple choices
for analytical methods and the jargon emerging along with these developments, real-time …

Reverse transcription quantitative PCR (RT-qPCR) is considered today as the gold standard
for accurate, sensitive and fast measurement of gene expression. Unfortunately, what many …

Abstract Design of primers and probes is one of the most crucial factors affecting the success
and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable …