This chapter analyzes the quantification strategies in real-time RT-PCR and all
corresponding markers of a successful real-time RT-PCR. The following aspects are …

Reverse transcription PCR (RT-PCR) represents a sensitive and powerful tool for analyzing
RNA. While it has tremendous potential for quantitative applications, a comprehensive …

Extensive detail on the application of the real‐time quantitative polymerase chain reaction
(QPCR) for the analysis of gene expression is provided in this unit. The protocols are …

Real‐time PCR is a recent modification to the polymerase chain reaction that allows precise
quantification of specific nucleic acids in a complex mixture by fluorescent detection of …

Real‐time PCR is a recent modification to the polymerase chain reaction that allows precise
quantification of specific nucleic acids in a complex mixture by fluorescent detection of …

Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse
transcribed from mRNA is on the way to becoming a routine tool in molecular biology to …

In this paper we determine the limits and accuracy of quantitative reverse transcription (RT)-
PCR using a modification of the original protocol. The quantification of mRNA with this …

Real-time PCR has become one of the most widely used methods of gene quantitation
because it has a large dynamic range, boasts tremendous sensitivity, can be highly …

The introduction of real-time PCR technology has significantly improved and simplified the
quantification of nucleic acids, and this technology has become an invaluable tool for many …

Quantitative real-time polymerase chain reaction (QRT-PCR) has become an extensively
applied technique. It enables quantitative analyses of gene expression applicable to basic …