To study the pathophysiology of human diseases, develop innovative treatments, and refine
approaches for regenerative medicine require appropriate preclinical models. Pigs share …

As the genetic mutations driving human disease are identified, there is an increasing need
for a biomedical model that can accurately represent the disease of interest and provide a …

Precise genome editing is a powerful tool for analysis of gene function. However, in
spermatogonial stem cells (SSCs), this still remains a big challenge mainly due to low …

Genome editing tools have revolutionized the generation of genetically modified animals
including livestock. In particular, the domestic pig is a proven model of human physiology …

Editing the genome to create specific sequence modifications is a powerful way to study
gene function and promises future applicability to gene therapy. Creation of precise …

The CRISPR/Cas9 system is a simpler and more versatile method compared to other
engineered nucleases such as Zinc Finger Nucleases (ZFNs) and Transcription Activator …

Genetically-modified domestic animal models are of increasing significance in biomedical
research and agriculture. As authentic ES cells derived from domestic animals are not yet …

Historically, genetic engineering in livestock proved to be challenging. Without stable
embryonic stem cell lines to utilize, somatic cell nuclear transfer (SCNT) had to be employed …

Spermatogonial stem cells (SSCs) can produce numerous male gametes after
transplantation into recipient testes, presenting a valuable approach for gene therapy and …

Mouse spermatogonial stem cells (SSCs) can be cultured for multiplication and maintained
for long periods while preserving their spermatogenic ability. Although the cultured SSCs …