The advent of targeted CRISPR-Cas nuclease technologies has revolutionized the ability to
perform precise genome editing in both established and emerging model systems. CRISPR …

Primordial germ cells (PGCs), the embryonic precursors of the sperm and egg, are used for
the introduction of genetic modifications into avian genome. Introduction of small defined …

Background Nuclease-based genome editing has rapidly sped the creation of new models
of human disease. These techniques also hold great promise for the future of clinical …

The use of animal models, particularly rodents, has been immensely important to nearly all
aspects of biomedical research, from basic science exploration to translational discoveries …

Recent advances in the study of the CRISPR/Cas9 system have provided a precise and
versatile approach for genome editing in various species. However, the applicability and …

Since the domestication of animals, livestock species are an important source of protein-rich
food, and other animal products. The very recent progress in genetic engineering allows to …

Engineering of the mouse germline is a key technology in biomedical research for studying
the function of genes in health and disease. Since the first knockout mouse was described in …

The ease and efficacy of CRISPR/Cas9 germline gene editing in animal models paved the
way to human germline gene editing (HGGE), by which permanent changes can be …

In the past few years, we have witnessed and been excited by biotechnological advances
that allow for the precise editing of genomic information in living eukaryotic cells. Facilitated …

Recent advances in genome editing have greatly improved knock-in (KI) efficiency–.
Searching for factors to further improve KI efficiency for therapeutic use and generation of …