Genetically-modified domestic animal models are of increasing significance in biomedical
research and agriculture. As authentic ES cells derived from domestic animals are not yet …

Transgenic technology has become an essential tool for the development of animal
biotechnologies, and animal cloning through somatic cell nuclear transfer (SCNT) enabled …

The ability to produce transgenic animals through the introduction of exogenous DNA has
existed for many years. However, past methods available to generate transgenic animals …

Genetic engineering (GE) of livestock initially has been accomplished primarily using
pronuclear microinjection into zygotes (1985–1996). The applications of the technology …

The ability to produce transgenic animals through the introduction of exogenous DNA has
existed for many years. However, past methods available to generate transgenic animals …

Contents Somatic cell nuclear transfer (SCNT) was first developed in livestock for the
purpose of accelerating the widespread use of superior genotypes. Although many …

To study the pathophysiology of human diseases, develop innovative treatments, and refine
approaches for regenerative medicine require appropriate preclinical models. Pigs share …

Domestic animals can be cloned using techniques such as embryo splitting and nuclear
transfer to produce genetically identical individuals. Although embryo splitting is limited to …

Historically, genetic engineering in livestock proved to be challenging. Without stable
embryonic stem cell lines to utilize, somatic cell nuclear transfer (SCNT) had to be employed …

Reprogramming of somatic DNA by oocytes is inadequate and thus often causes an
abnormal epigenetic status in embryos derived from somatic cell nuclear transfer (SCNT) …