The production of transgenic animals has many biotechnological applications; however,
available techniques still remain inefficient. This review summarizes the contributions of our …

Genome editing in pigs has tremendous practical applications for biomedicine. The advent
of genome editing technology, with its use of site‐specific nucleases—including ZFNs …

The demand for food will increase to an unprecedented level over the next 30 years owing
to human population expansion, thus necessitating an evolution that improves the efficiency …

Serial cloning by somatic cell nuclear transfer (SCNT) is a critical tool for the expansion of
precious transgenic lines or resetting the lifespan of primary transgenic cells for multiple …

This study explored the possibility of producing transgenic cloned embryos by interspecies
somatic cell nuclear transfer (iSCNT) of cattle, mice, and chicken donor cells into enucleated …

REPRODUCTION Page 1 © 2021 Society for Reproduction and Fertility https://doi.org/10.1530/REP-21-0072
ISSN 1470–1626 (paper) 1741–7899 (online) Online version via https://rep.bioscientifica.com …

Manipulation of the pig genome is currently restricted to the random insertion of new DNA
using pronuclear microinjection. This method suffers from a number of inherent limitations …

Somatic cell nuclear transfer (SCNT) has become a unique and powerful tool for epigenetic
reprogramming research and gene manipulation in animals. Although the success rates of …

For many years the lack of germline competent embryonic stem cell lines in livestock meant
that the targeted modification of endogenous genes was not possible in these species. The …

Transplantation of germ cells from fertile donor mice to the testes of infertile recipient mice
results in donor-derived spermatogenesis and transmission of the donor's genetic material …