FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways
DNA damage-activated signaling pathways are critical for coordinating multiple cellular
processes, which must be tightly regulated to maintain genome stability. To provide a …
processes, which must be tightly regulated to maintain genome stability. To provide a …
Revolutionizing DNA repair research and cancer therapy with CRISPR–Cas screens
All organisms possess molecular mechanisms that govern DNA repair and associated DNA
damage response (DDR) processes. Owing to their relevance to human disease, most …
damage response (DDR) processes. Owing to their relevance to human disease, most …
CRISPR/CAS9-based DNA damage response screens reveal gene-drug interactions
DNA damage response (DDR) is critically important for cell survival, genome maintenance,
and its defect has been exploited therapeutically in cancer treatment. Many DDR-targeting …
and its defect has been exploited therapeutically in cancer treatment. Many DDR-targeting …
Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases
H Huang, Y Hu, G Huang, S Ma, J Feng… - Communications …, 2021 - nature.com
Genome-wide identification of DNA double-strand breaks (DSBs) induced by CRISPR-
associated protein (Cas) systems is vital for profiling the off-target events of Cas nucleases …
associated protein (Cas) systems is vital for profiling the off-target events of Cas nucleases …
Mapping cellular responses to DNA double-strand breaks using CRISPR technologies
DNA double-strand breaks (DSBs) are one of the most genotoxic DNA lesions, driving a
range of pathological defects from cancers to immunodeficiencies. To combat genomic …
range of pathological defects from cancers to immunodeficiencies. To combat genomic …
Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites
Here we present an approach that combines a clustered regularly interspaced short
palindromic repeats (CRISPR) system that simultaneously targets hundreds of …
palindromic repeats (CRISPR) system that simultaneously targets hundreds of …
[HTML][HTML] Genome-scale mapping of DNA damage suppressors through phenotypic CRISPR-Cas9 screens
To maintain genome integrity, cells must accurately duplicate their genome and repair DNA
lesions when they occur. To uncover genes that suppress DNA damage in human cells, we …
lesions when they occur. To uncover genes that suppress DNA damage in human cells, we …
From DNA break repair pathways to CRISPR/Cas-mediated gene knock-in methods
MR Bazaz, H Dehghani - Life sciences, 2022 - Elsevier
Various DNA breaks created via programmable CRISPR/Cas9 nuclease activity results in
different intracellular DNA break repair pathways. Based on the cellular repair pathways …
different intracellular DNA break repair pathways. Based on the cellular repair pathways …
CRISPR/Cas9-induced DNA damage enriches for mutations in a p53-linked interactome: implications for CRISPR-based therapies
Inactivating p53 mutations are the most abundant genetic alterations found in cancer. Here
we show that CRISPR/Cas9-induced double-stranded DNA breaks enrich for cells deficient …
we show that CRISPR/Cas9-induced double-stranded DNA breaks enrich for cells deficient …
A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
Double-strand breaks (DSBs) are the most toxic type of DNA lesions. Cells repair these
lesions using either end protection-or end resection-coupled mechanisms. To study DSB …
lesions using either end protection-or end resection-coupled mechanisms. To study DSB …