Construção de biossensor para detecção de compostos BTEX baseado em fosfatase alcalina sob regulação xylR/Pu e avaliação de sua regulação metabólica.

AA Baceti - 2010 - teses.usp.br
Este projeto teve como objetivo a construção de biosensores para a detecção de compostos
monoaromáticos do grupo BTEX a partir dos componentes da via de degradação de …

A microbial sensor for organophosphate hydrolysis exploiting an engineered specificity switch in a transcription factor

RK Jha, TL Kern, Y Kim, C Tesar… - Nucleic Acids …, 2016 - academic.oup.com
A whole-cell biosensor utilizing a transcription factor (TF) is an effective tool for sensitive and
selective detection of specialty chemicals or anthropogenic molecules, but requires access …

Development of exobiopolymer-based biosensor for detection of phosphate in water.

TK Taranpreet Kaur, AG Abhijit Ganguli… - 2013 - cabidigitallibrary.org
The present study was conducted to develop a biosensor by exploiting phosphate-binding
capacity of exobiopolymer (EBP) produced by Acinetobacter sp. An environmental isolate of …

Phosphate binding protein as the biorecognition element in a biosensor for phosphate

LLE Salins, SK Deo, S Daunert - Sensors and Actuators B: Chemical, 2004 - Elsevier
This work explores the potential use of a member of the periplasmic family of binding
proteins, the phosphate binding protein (PBP), as the biorecognition element in a sensing …

Construction and comparison of Escherichia coli whole-cell biosensors capable of detecting aromatic compounds

MN Kim, HH Park, WK Lim, HJ Shin - Journal of microbiological methods, 2005 - Elsevier
The XylR regulatory protein is a transcription factor involved in the BTEX (benzene, toluene,
ethylbenzene, and xylene) degradation pathway in Pseudomonas species. When XylR …

Development of exobiopolymer-based biosensor for detection of phosphate in water

T Kaur, A Ganguli, M Ghosh - Water science and technology, 2013 - iwaponline.com
The present study was conducted to develop a biosensor by exploiting phosphate-binding
capacity of exobiopolymer (EBP) produced by Acinetobacter sp. An environmental isolate of …

Switching the Ligand Specificity of the Biosensor XylS from meta to para-Toluic Acid through Directed Evolution Exploiting a Dual Selection System

Y Ogawa, Y Katsuyama, K Ueno… - ACS Synthetic …, 2019 - ACS Publications
The Pseudomonas putida transcriptional activator XylS induces transcription from the Pm
promoter in the presence of several benzoic acid effectors, with m-toluic acid being the most …

Identificação em bases moleculares de genes de Burkholderia sacchari envolvidos no catabolismo de propianato via α-oxidação.

ACC Lemos - 2017 - teses.usp.br
Burkholderia sacchari é uma espécie de bactéria capaz de acumular polihidroxialcanoatos
em condições de limitação de um nutriente essencial e excesso de fonte de carbono. A …

Microbial biosensors for organophosphate pesticides

A Mulchandani, Rajesh - Applied biochemistry and biotechnology, 2011 - Springer
Organophosphates, amongst the most toxic substance known, are used widely in agriculture
around the world. Their extensive use, however, has resulted in their occurrence in the water …

Engineering whole-cell biosensors with no antibiotic markers for monitoring aromatic compounds in the environment

A de Las Heras, V de Lorenzo - Microbial Metabolic Engineering: Methods …, 2012 - Springer
A cornerstone of Synthetic Biology is the engineering of gene regulatory networks.
Construction of such biological circuits has been used not only to elucidate the dynamics of …