We report new progress on the use of the rolling circle transcription strategy for production of
ribozymes in vitro and in living cells. Optimized circular DNA vectors have been selected …

Ribozymes can cleave RNA sequences specifically by recognition of their target RNA
through Watson-Crick base pair formation to form the substrate-ribozyme complex. Thus …

As experimental methods in molecular biology have rapidly progressed, the need for simple
methodology to create RNA size markers has become increasingly apparent. Ribosomal …

The completely unexpected discovery that the RNA molecule has catalytic properties (1, 2)
has led to a plethora of interest in the identification and utilization of a variety of catalytic …

Inhibition of gene expression is an important experimental method to determine the function
of genes, and could have a great impact upon the treatment of diseases in which gene …

The ability of certain enzymatic RNA molecules, or ribozymes, to site-specifically cleave
other RNA molecules opens new vistas in gene therapy. Ribozymes can be designed to …

We describe experiments aimed at establishing whether circular single-stranded DNAs can
form promoters for bacterial transcription from small folded motifs. In vitro selection …

The hairpin ribozyme has been developed and designed to target and cleave efficiently
heterologous RNA sequences in a trans-reaction (1–3). Heterologous RNA cleavage has …

It was only in the early 1980s that the first catalytic RNA molecules (ribozymes) were
identified. A few years later, the book Therapeutic Applications of Ribozymes has been …

A simple new strategy for the in vitro synthesis of circular RNAs and hairpin ribozymes is
described. Circular single-strand DNA oligonucleotides 67–79 nt in length are constructed to …