Whole genome wide analysis of transcription using RNA-Seq methods is a powerful way to
elucidate differential expression of gene features in bacteria across different conditions as …

Genome-wide studies of bacterial gene expression are shifting from microarray technology
to second generation sequencing platforms. RNA-seq has a number of advantages over …

We provide a detailed protocol for preparing cDNA libraries suitable for high-throughput
sequencing that are derived specifically from the 5′ ends of RNA (5′ specific RNA-seq) …

Recent advances in high-throughput RNA sequencing (RNA-seq) have enabled
tremendous leaps forward in our understanding of bacterial transcriptomes. However …

Owing to the complexities of bacterial RNA biology, the transcriptomes of even the best
studied bacteria are not fully understood. To help elucidate the transcriptional landscape of …

As part of the ongoing renaissance of phage biology, more phage genomes are becoming
available through DNA sequencing. However, our understanding of the transcriptome …

Background High-throughput sequencing of cDNA libraries (RNA-Seq) has proven to be a
highly effective approach for studying bacterial transcriptomes. A central challenge in …

RNA sequencing has become the method of choice to study the transcriptional landscape of
phage-infected bacteria. However, short-read RNA sequencing approaches generally fail to …

RNA sequencing (RNA‐seq) has become the preferred method for global quantification of
bacterial gene expression. With the continued improvements in sequencing technology and …

The ability to simultaneously characterize the bacterial and host expression programs during
infection would facilitate a comprehensive understanding of pathogen–host interactions …