We developed a system using 'passive'reporter genes driven by aleurone tissue-specific
promoters to test the effect of methylation of foreign DNA on its stability after introduction into …

The stable transformation of crop plants belonging to the Gramineae family is of great
importance for all further genetic manipulation. Since agrobacteria do not work (efficiently) …

Instability of transgene expression in plants is often associated with complex multicopy
patterns of transgene integration at the same locus, as well as position effects due to random …

DNA methylation is known to play a crucial role in regulating plant development and organ
or tissue differentiation. Here, we focused on the DNA methylation dynamics during the …

Production of transgenic plants is now routine for many of our crop species. Methods for the
detailed molecular analysis of transgenic plants are available, but often the exact location of …

In order to evaluate the long-term stability of transgene expression driven by the B 1-and D-
hordein promoters in transgenic barley (Hordeum vulgare L., 2 n= 2 x= 14), we analyzed …

Plant DNA is known to contain 5-methylcytosine as the only modified base. The methylation
of plant DNA has been shown to take place exclusively at CpG and CpXpG sequences [1]. In …

A number of stably transformed wheat cell lines were obtained using two different direct
gene transfer techniques. When integration of the foreign genes was investigated in DNA …

Sequencing across the junction between an integrated transfer DNA (T-DNA) and a host
plant genome provides two important pieces of information. The junctions themselves …

DNA methylation is a chromatin modification that is often associated with the exciting and
sometimes unpredictable patterns of inheritance that can unfold with epigenetic …