Tetracycline (TC)-sensing bioreporters using green fluorescent protein (GFP) were
generated in Escherichia coli and solvent-tolerant Acinetobacter oleivorans. A TC-inducible …

Tetracycline (TC)-sensing bioreporters using green fluorescent protein (GFP) were
generated in Escherichia coli and solvent-tolerant Acinetobacter oleivorans. A TC-inducible …

Three different mini-Tn 5 plasmids, containing a tetracycline-inducible promoter, P tet and a
regulatory gene, tetR, in operon fusions with a reporter gene system (lacZYA, luxCDABE or …

An extended range whole-cell tetracycline biosensor strain was constructed by insertion of
the tet (M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid …

Three separate genetic strategies, based upon the induced expression of three different
genes (lacZ, selA and nuoA) were tested to provide the SciTox assay with sensitive and …

A plasmid containing a transcriptional fusion between fet regulated fet promoter from
plasmid pOT182 and Enhanced Green Fluorescent Protein (EGFP) gene was created and …

The bacterial respiratory gene, nuoA, was previously used as a reporter gene in an
amperometric, whole cell biosensor for tetracycline (Tet) detection. While the nuoA-based …

Tetracycline repressor gene (tetR) from E. coli transposon Tn10 was fused in frame with
green fluorescent protein gene (gfp) from jellyfish Aequorea Victoria on an E. coli expression …

Materials and Methods Mouse embryo fibroblast 10T1/2 cells in a 35 mm culture dish were
transfected with 2 μg of pcDNA6/TR plasmid DNA (Invitrogen, Carlsbad, CA, USA) with …

An Escherichia coli biosensor strain, harboring the plasmid pTGFP2, was introduced into the
gastrointestinal tract of gnotobiotic rats that continuously received drinking water containing …