Preparation of RNA from Bacteria Infected with Bacteriophages: A Case Study from the Marine Unicellular Synechococcus sp. WH7803 Infected by Phage S-PM2

J Shan, M Clokie - Bacteriophages: Methods and Protocols, Volume 2 …, 2009 - Springer
Bacteriophages manipulate bacterial gene expression in order to express their own genes
or influence bacterial metabolism. Gene expression can be studied using real-time PCR or …

Quantification of host and phage mRNA expression during infection using real-time PCR

MRJ Clokie - Bacteriophages: Methods and Protocols, Volume 2 …, 2009 - Springer
Real-time, or quantitative PCR, is a valuable technique useful in bacteriophage research to
quantify the abundance of phage or host gene transcripts. It can be used during the infection …

Bacteriophages: Methods and protocols-Volume III

MRJ Clokie, AM Kropinski… - Methods in Molecular …, 2018 - compendiumkustenzee.be
This book expands on the previous volumes with new chapters exploring emerging themes
and methodologies in bacterial virus research. The chapters in this book are divided into 4 …

Nucleic acids from the host bacterium as a major source of nucleotides for three marine bacteriophages

J Wikner, JJ Vallino, GF Steward… - FEMS microbiology …, 1993 - academic.oup.com
The incorporation of 32P-phosphorus into marine bacteriophage nucleic acid was studied in
culture experiments to investigate the source of nucleotides used by the phage. We …

Single-stranded RNA bacteriophages

J Van Duin - The bacteriophages, 1988 - Springer
Since their discovery in 1961 by Loeb and Zinder, the RNA phages have served as a model
system to explore a variety of problems in molecular biology. As a source of homogeneous …

Nearly identical bacteriophage structural gene sequences are widely distributed in both marine and freshwater environments

CM Short, CA Suttle - Applied and environmental microbiology, 2005 - Am Soc Microbiol
Primers were designed to amplify a 592-bp region within a conserved structural gene (g20)
found in some cyanophages. The goal was to use this gene as a proxy to infer genetic …

[图书][B] Bacteriophages

MRJ Clokie, AM Kropinski, R Lavigne - 2009 - Springer
We are increasingly aware of the many and varied roles that bacteriophages play in
microbial ecology and evolution. The implications of bacteriophage–bacteria interactions …

[HTML][HTML] Bacteriophages as sources of small non-coding RNA molecules

S Bloch, N Lewandowska, G Węgrzyn… - Plasmid, 2021 - Elsevier
Bacteriophages play an essential role in the transferring of genes that contribute to the
bacterial virulence and whose products are dangerous to human health. Interestingly …

[HTML][HTML] Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within …

SD Wharam, MJ Hall, WH Wilson - Virology journal, 2007 - Springer
Abstract Background Signal-Mediated Amplification of RNA Technology (SMART) is an
isothermal nucleic acid amplification technology, developed for the detection of specific …

[PDF][PDF] RNA bacteriophages: diversity, abundance, and applications

J Callanan - 2021 - cora.ucc.ie
The number of novel bacteriophage sequences has expanded significantly as a result of
many metagenomic studies of phage populations in diverse environments. Most of these …