Conventional gene targeting is widely used to generate knockout mice. However, the
conventional method depends on a rare event, spontaneous homologous recombination …

Targeted genome editing has opened new frontiers to study gene functions by introducing
alterations in the genome of an organism. The process incorporates deletion, insertion, or …

Background Genome editing technology heralds a new era for animal cell engineering.
Programmable site-specific nucleases, such as transcription activator-like effector nucleases …

Genome editing represents a group of newly developed capabilities that allow nucleotide-
level targeting of genomic regions in a wide range of species. This chapter provides an …

After induced mutagenesis and transgenesis, genome editing is the next step in the
development of breeding techniques. Genome editing using site-directed nucleases …

Genome modification technologies are powerful tools for molecular biology and related
areas. Advances in animal transgenesis and genome editing technologies during the past …

Genome editing is a powerful technology that can efficiently alter the genome of organisms
to achieve targeted modification of endogenous genes and targeted integration of …

It has recently become commonplace to editorialize on the extent to which genome editing
has transformed modern biological research and perhaps, in the future, biomedicine …

Contents In the last decade, the research community has witnessed a blooming of targeted
genome editing tools and applications. Novel programmable DNA nucleases such as zinc …

Knock-in mice are useful for evaluating endogenous gene expressions and functions in vivo.
Instead of the conventional gene-targeting method using embryonic stem cells, an …