In the past few years, new technologies have arisen that enable higher efficiency of gene
editing. With the increase ease of using gene editing technologies, it is important to consider …

The Transgenic livestock can be useful for the production of disease-resistant animals, pigs
for xenotranplantation, animal bioreactor for therapeutic recombinant proteins and disease …

The production of transgenic farm animals (eg, cattle) via genome engineering for the gain
or loss of gene functions is an important undertaking. In the initial stages of genome …

Genome editing is a powerful technology that can efficiently alter the genome of organisms
to achieve targeted modification of endogenous genes and targeted integration of …

Historically, genetic engineering in livestock proved to be challenging. Without stable
embryonic stem cell lines to utilize, somatic cell nuclear transfer (SCNT) had to be employed …

In the recent years, sequence-specific nucleases such as ZFNs, TALENs, and CRISPR/Cas9
have revolutionzed the fields of animal genome editing and transgenesis. However, these …

Due to the lack of germline transmitting pluripotent stem cells (PSCs) cell lines and the
extreme difficulty of somatic cell nuclear transfer (SCNT) in rabbit, the gene targeting …

Conventional gene targeting is widely used to generate knockout mice. However, the
conventional method depends on a rare event, spontaneous homologous recombination …

Genetically modified animals, especially rodents, are widely used in biomedical research.
However, non-rodent models are required for efficient translational medicine and preclinical …

Genome editing tools (GET), including zinc-finger nucleases (ZFN), transcription activator-
like endonucleases (TALENS), and meganucleases possess long recognition sites and are …