Live pigs produced from genome edited zygotes
SG Lillico, C Proudfoot, DF Carlson, D Stverakova… - Scientific reports, 2013 - nature.com
Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN)
genome editing technology enables site directed engineering of the genome. Here we …
genome editing technology enables site directed engineering of the genome. Here we …
Genetic modification of animals in the next century
JD Murray - Theriogenology, 1999 - Elsevier
Since the initial demonstration in 1982 of profound phenotypic effects stemming from the
expression of a single transgene, genetic engineering has revolutionized fundamental …
expression of a single transgene, genetic engineering has revolutionized fundamental …
Gene targeting, genome editing: from Dolly to editors
W Tan, C Proudfoot, SG Lillico, CBA Whitelaw - Transgenic research, 2016 - Springer
One of the most powerful strategies to investigate biology we have as scientists, is the ability
to transfer genetic material in a controlled and deliberate manner between organisms. When …
to transfer genetic material in a controlled and deliberate manner between organisms. When …
Somatic cell nuclear transfer followed by CRIPSR/Cas9 microinjection results in highly efficient genome editing in cloned pigs
The domestic pig is an ideal “dual purpose” animal model for agricultural and biomedical
research. With the availability of genome editing tools such as clustered regularly …
research. With the availability of genome editing tools such as clustered regularly …
Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats
Z Lu, L Zhang, Q Mu, J Liu, Y Chen, H Wang, Y Zhang… - Agriculture, 2024 - mdpi.com
Due to recent innovations in gene editing technology, great progress has been made in
livestock breeding, with researchers rearing gene-edited pigs, cattle, sheep, and other …
livestock breeding, with researchers rearing gene-edited pigs, cattle, sheep, and other …
Genome editing and genetic engineering in livestock for advancing agricultural and biomedical applications
Genetic modification of livestock has a longstanding and successful history, starting with
domestication several thousand years ago. Modern animal breeding strategies …
domestication several thousand years ago. Modern animal breeding strategies …
GONAD: a novel CRISPR/Cas9 genome editing method that does not require ex vivo handling of embryos
CB Gurumurthy, G Takahashi, K Wada… - Current protocols in …, 2016 - Wiley Online Library
Transgenic technologies used for creating a desired genomic change in animals involve
three critical steps: isolation of fertilized eggs, microinjection of transgenic DNA into them …
three critical steps: isolation of fertilized eggs, microinjection of transgenic DNA into them …
In Vitro Validation of Transgene Expression in Gene-Edited Pigs Using CRISPR Transcriptional Activators
KM Polkoff, J Chung, SG Simpson, K Gleason… - The CRISPR …, 2020 - liebertpub.com
The use of CRISPR-Cas and RNA-guided endonucleases has drastically changed research
strategies for understanding and exploiting gene function, particularly for the generation of …
strategies for understanding and exploiting gene function, particularly for the generation of …
Enhancing animal disease resistance, production efficiency, and welfare through precise genome editing
Z Liu, T Wu, G Xiang, H Wang, B Wang, Z Feng… - International Journal of …, 2022 - mdpi.com
The major goal of animal breeding is the genetic enhancement of economic traits. The
CRISPR/Cas system, which includes nuclease-mediated and base editor mediated genome …
CRISPR/Cas system, which includes nuclease-mediated and base editor mediated genome …
Genome editing in large animals: current status and future prospects
Large animals (non-human primates, livestock and dogs) are playing important roles in
biomedical research, and large livestock animals serve as important sources of meat and …
biomedical research, and large livestock animals serve as important sources of meat and …