The theory of a method for determining active concentration of nonpurified protein samples
using the BIAcore biosensor technology has been developed. The method relies on change …

Using biosensor technology, it is possible to measure protein concentration when the
binding of the protein to an appropriate ligand immobilized on the sensor surface is totally …

Binding data obtained with Biacore instrumentation is often evaluated using a kinetic
transport model where reaction rate constants and a mass transport coefficient are used to …

Numerical simulation of BIAcore sensorgrams has highlighted the need for concern about
an assumption, inherent in current determinations of rate constants for macromolecular …

Surface-based binding assays are often influenced by the transport of analyte to the sensor
surface. Using simulated data sets, we test a simple two-compartment model to see if its …

The transport and kinetic processes describing biomolecular interactions in the BIACORE
optical biosensor have been studied with the help of a mathematical model. In comparison …

To explore the variability in biosensor studies, 150 participants from 20 countries were given
the same protein samples and asked to determine kinetic rate constants for the interaction …

The mass-transfer processes that affect kinetic measurements of biospecific interactions
between one species in a flowing solution and another species immobilized in a thin …

Optical biosensors, including the BIACORE, provide an increasingly popular method for
determining reaction rates of biomolecules. In a flow chamber, with one reactant …

A methodology using biosensor technology for combined kinetic and thermodynamic
analysis of biomolecular interactions is described. Rate and affinity constants are …