The influence of reaction order and external mass transfer limitations on the binding kinetics
of antigen in solution to antibody covalently or non-covalently attached to a cylindrical fiber …

A range of studies were carried out to investigate the underlying reason for differences in
dynamic binding capacities observed with various antibodies and Fc‐fusion proteins during …

Macromolecular interactions observed using surface plasmon resonance technology
(BIAcore, Pharmacia) often display kinetic behavior which deviates from the pseudo-first …

The use of a simple device by which a layer of solvent may be deposited onto a solution of
an optically absorbing solute in a cylindrical quartz tube, without substantial mixing of …

Using BIAcore™ technology, we determined the rate constants for a protein antigen-
antibody interaction that was mass transport limited on the optical biosensor. The antigen …

A commercial surface plasmon resonance biosensor, BIACORE X, is employed as a
detector in a closed loop of a small sample volume. The sample is continuously circulated by …

The kinetics of antibody-binding to surface-immobilized antigen was studied by ellipsometry,
using bovine serum albumin as antigen. The results show that the initial binding of antibody …

Aspersion Is minimized by the low dead volume In the system (= 0.4 pi) accomplished by
using Integrated sample loops and thin conduits. An asset of this Integration Is the low …

Reliable kinetic estimates can be obtained from significantly less data than is commonly
used today, particularly in the characterization of 1: 1 interactions involving low molecular …

Many techniques rely on the binding activity of surface-immobilized proteins, including
antibody-based affinity biosensors for the detection of analytes, immunoassays, protein …