Many techniques rely on the binding activity of surface-immobilized proteins, including
antibody-based affinity biosensors for the detection of analytes, immunoassays, protein …

We demonstrate that the interaction between myoglobin and an immobilized anti-myoglobin
antibody measured on BIAcore 2000 can be described by a simple bimolecular reaction …

The binding kinetics recorded for many interactions using BIAcore and IAsys optical
biosensors do not fit a simple bimolecular interaction model (A+ B⇄ AB). Three methods of …

Abstract Surface Plasmon Resonance biosensors measure the interaction between a
molecule in solution and its interaction partner attached to a sensor surface. Under certain …

The introduction of affinity-based biosensors has permitted label-free functional analysis of
biomolecular interactions in real time. A variety of methods are now based on BIACORE® …

The influence of reaction-order and external mass-transfer limitations on the binding kinetics
of antigen in solution to antibody covalently attached to a cylindrical fiber-optic biosensor is …

The use of confocal scanning laser microscopy (CSLM) has recently been described for the
visualization of intraparticle protein profiles during single‐protein finite bath uptake …

Many biotechnological applications use protein receptors immobilized on solid supports.
Although, in solution, these receptors display homogeneous binding affinities and …

Optical biosensors are finding increasing use in the determination of kinetic and equilibrium
constants for a variety of biomolecular interactions. Usually these biosensors require one …

Porous substrates have gained widespread interest for biosensor applications based on
molecular recognition. Thus, there is a great demand to systematically investigate the …