Benefits and limitations of porous substrates as biosensors for protein adsorption

TD Lazzara, I Mey, C Steinem, A Janshoff - Analytical chemistry, 2011 - ACS Publications
Porous substrates have gained widespread interest for biosensor applications based on
molecular recognition. Thus, there is a great demand to systematically investigate the …

Determination of association rate constants by an optical biosensor using initial rate analysis

PR Edwards, RJ Leatherbarrow - Analytical biochemistry, 1997 - Elsevier
We show that initial rate analysis can be successfully applied to analyze experimental
binding data generated by an optical biosensor. The initial rates of binding obtained from …

Analysis of mass transport-limited binding kinetics in evanescent wave biosensors

P Schuck, AP Minton - Analytical biochemistry, 1996 - Elsevier
It is shown that currently used methods for analyzing surface plasmon resonance or
resonant mirror biosensor data do not adequately take into account the effects of mass …

[PDF][PDF] BIACORE: an affinity biosensor system for characterization of biomolecular interactions

M Malmqvist - Biochemical society transactions, 1999 - researchgate.net
With the introduction of affinity biosensors for biomolecular interactions analysis (BIA) in
1990, a new concept for analysis of the functional properties of biomolecules was introduced …

Continuous measurements of a binding reaction using a capacitive biosensor

M Hedström, IY Galaev, B Mattiasson - Biosensors and Bioelectronics, 2005 - Elsevier
A capacitive biosensor with polyclonal antibodies raised against human serum albumin
(HSA) immobilized on a gold transducer has been developed for continuous measurement …

Kinetics of protein-protein interactions at the surface of an optical biosensor

PR Edwards, A Gill, DV Pollardknight, M Hoare… - Analytical …, 1995 - Elsevier
Methods based on the use of optical biosensors have recently become available to provide
a convenient means of determining the rate and equilibrium constants for bimolecular …

[引用][C] Determination of effective diffusion coefficients—An important parameters for the efficiency of immobilized biocatalysts

P Grunwald - Biochemical education, 1989 - Elsevier
The limitation of mass transport by diffusion plays an important role in heterogeneous
catalysis especially when the insoluble catalyst has a porous structure. Therefore …

Modeling Taylor dispersion injections: determination of kinetic/affinity interaction constants and diffusion coefficients in label-free biosensing

JG Quinn - Analytical biochemistry, 2012 - Elsevier
A new method based on Taylor dispersion has been developed that enables an analyte
gradient to be titrated over a ligand-coated surface for kinetic/affinity analysis of interactions …

Future trends in biosensor research

PJ O'Connell, GG Guilbault - Analytical letters, 2001 - Taylor & Francis
The past few years have seen a dramatic change in biosensor technology. This change can
be attributed to the convergence of three different strands of research: a. Researchers have …

Comparative analyses of a small molecule/enzyme interaction by multiple users of Biacore technology

MJ Cannon, GA Papalia, I Navratilova, RJ Fisher… - Analytical …, 2004 - Elsevier
To gauge the experimental variability associated with Biacore analysis, 36 different
investigators analyzed a small molecule/enzyme interaction under similar conditions …