A method is proposed for identification of kinetic parameters when diffusion of substrates is
limiting in reactions catalyzed by immobilized enzymes. This method overcomes …

Dynamic models that could describe the adsorption of adsorbate onto ligand immobilized on
porous or non-porous particles in batch and column systems, are presented and solved …

Surface plasmon resonance detection makes it possible to directly measure the binding of
an analyte in solution to a surface immobilized binding partner. The immobilization of one …

Publisher Summary This chapter discusses the analysis of the kinetic behavior of enzymes
entrapped in or bound to membranes or other supports, as can be observed from …

Surface plasmon resonance (SPR) allows for the investigation of the functional nature of
binding interactions and provides detailed kinetic information across a wide range of …

Biolayer interferometry is a novel method for quantifying macromolecules, such as proteins,
in solution. The presence of other, non-binding molecules does not interfere with …

Publisher Summary This chapter discusses the kinetic behavior of immobilized enzyme
systems. In the case of immobilized enzymes, the kinetic behavior can be controlled by both …

In the Biacore biosensor, a widely used tool for studying the kinetics of ligand/receptor
binding, receptors are commonly localized to the sensor surface through attachment to …

A general model is presented and used to predict the dynamic behavior of the adsorption
and wash stages of biospecific adsorption (affinity chromatography) in a finite bath. The …

It is very important to understand the equilibrium and dynamic characteristics of biospecific
adsorption (affinity chromatography) for both scientific and application purposes …