Rapid detection of NDM and VIM carbapenemase encoding genes by recombinase polymerase amplification and lateral flow–based detection

AKS Ayfan, J Macdonald, PNA Harris, C Heney… - European Journal of …, 2021 - Springer
Carbapenemase-producing organisms (CPOs) pose a serious clinical threat and rapid
detection tools are essential to aid in patient management. We developed rapid and simple …

[HTML][HTML] Fast and reliable detection of carbapenemase genes in various Gram negatives using a new commercially available fluorescence-based real-time …

M Sadek, A Demord, L Poirel, P Nordmann - Diagnostic microbiology and …, 2020 - Elsevier
Abstract The Revogene® Carba C assay is a real-time polymerase chain reaction–based
assay that runs on the microfluidic Revogene platform. It was recently designed for the …

Evaluation of the SpeeDx Carba (beta) multiplex real-time PCR assay for detection of NDM, KPC, OXA-48-like, IMP-4-like and VIM carbapenemase genes

A Bordin, E Trembizki, M Windsor, R Wee, LY Tan… - BMC Infectious …, 2019 - Springer
Abstract Background Carbapenemase-producing organisms (CPOs) have emerged as
antibiotic-resistant bacteria of global concern. Here we assessed the performance of the …

Multicentre evaluation of a real-time PCR assay to detect genes encoding clinically relevant carbapenemases in cultured bacteria

MJ Ellington, J Findlay, KL Hopkins, D Meunier… - International journal of …, 2016 - Elsevier
The performance and portability of a multiplex real-time PCR assay to detect KPC, NDM,
OXA-48-like and VIM carbapenemase gene families from bacterial isolates was assessed …

Multiplex PCR for detection of acquired carbapenemase genes

L Poirel, TR Walsh, V Cuvillier, P Nordmann - Diagnostic microbiology and …, 2011 - Elsevier
A rapid and reliable PCR-based technique was developed for detection of genes encoding
carbapenemases belonging to different classes. Primers were designed to amplify the …

Multi-centre evaluation of real-time multiplex PCR for detection of carbapenemase genes OXA-48, VIM, IMP, NDM and KPC

A van der Zee, L Roorda, G Bosman, AC Fluit… - BMC infectious …, 2014 - Springer
Background Resistance to carbapenem antibiotics is emerging worldwide among
Enterobacteriaceae. To prevent hospital transmission due to unnoticed carriage of …

[HTML][HTML] Multicenter evaluation of Xpert Carba-R assay for detection and identification of the carbapenemase genes in rectal swabs and clinical isolates

X Jin, H Zhang, S Wu, X Qin, P Jia, FC Tenover… - The Journal of Molecular …, 2021 - Elsevier
Rapid detection of carbapenemase-producing organisms is clinically desirable for hospital
infection control and antibiotic stewardship. In this multicenter study, the Xpert Carba-R …

A multiplex real-time PCR for the direct, fast, economic and simultaneous detection of the carbapenemase genes blaKPC, blaNDM, blaVIM and blaOXA-48

D Weiß, I Engelmann, SD Braun, S Monecke… - Journal of …, 2017 - Elsevier
A novel multiplex real-time PCR was designed to detect the clinically most important
carbapenemase genes, blaKPC, blaVIM, blaNDM and bla OXA-48. The multiplex assay was …

Detection of carbapenemase-producers: Evaluating the performance of the carbapenem inactivation method and Carba NP test versus multiplex PCR

LA Madkour, MS Soliman, DM Hassan… - Journal of Global …, 2017 - Elsevier
Objectives With a worrisome surge of carbapenem-resistant bacterial isolates, the diagnostic
arsenal has become in dire need of affordable and timely assays to detect the rapidly …

Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system

M Hofko, A Mischnik, M Kaase… - Journal of Clinical …, 2014 - Am Soc Microbiol
We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD
Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated …