Formamide-free genomic in situ hybridization allows unambiguous discrimination of highly similar parental genomes in diploid hybrids and allopolyploids

TS Jang, H Weiss-Schneeweiss - Cytogenetic and genome research, 2016 - karger.com
Polyploidy and hybridization play an important role in plant diversification and speciation.
The application of genomic in situ hybridization (GISH) allows the identification of parental …

[PDF][PDF] Genomic in situ hybridization in plants

GS Silva, MM Souza - Genet Mol Res, 2013 - funpecrp.com.br
Genomic in situ hybridization (GISH), which is a modification of fluorescent in situ
hybridization, has been widely used in the study of plants. It has become one of the most …

New horizons of genomic in situ hybridization

M Markova, B Vyskot - Cytogenetic and genome research, 2009 - karger.com
This review summarizes conventional and recent applications of genomic in situ
hybridization (GISH). GISH is a well recognized technique, but its modifications and …

Application of genomic in situ hybridization in horticultural science

F Ramzan, A Younis, KB Lim - International journal of genomics, 2017 - Wiley Online Library
Molecular cytogenetic techniques, such as in situ hybridization methods, are admirable tools
to analyze the genomic structure and function, chromosome constituents, recombination …

Application of GISH to characterize woody ornamental hybrids with small genomes and chromosomes

K Van Laere, L Khrustaleva… - Plant …, 2010 - Wiley Online Library
With 2 figures and 1 table Abstract Genomic in situ hybridization (GISH) is a powerful tool in
distinguishing parental genomes in plant hybrids. However, in plants with small genome …

GISH: resolving interspecific and intergeneric hybrids

N Piperidis - Molecular Plant Taxonomy: Methods and Protocols, 2014 - Springer
Genomic in situ hybridization (GISH) is an invaluable cytogenetic technique which enables
the visualization of whole genomes in hybrids and polyploidy taxa. Total genomic DNA from …

[HTML][HTML] Review of the application of modern cytogenetic methods (FISH/GISH) to the study of reticulation (polyploidy/hybridisation)

M Chester, AR Leitch, PS Soltis, DE Soltis - Genes, 2010 - mdpi.com
The convergence of distinct lineages upon interspecific hybridisation, including when
accompanied by increases in ploidy (allopolyploidy), is a driving force in the origin of many …

GISH technology in plant genome research

SN Raina, V Rani - Chromosome Painting: Principles, Strategies and …, 2001 - Springer
Genomic in situ hybridization, a modification of fluorescence in situ hybridization technology,
is one of the most exciting and versatile research tools to be developed in recent years. It …

Insight into octoploid strawberry (Fragaria) subgenome composition revealed by GISH analysis of pentaploid hybrids

B Liu, EG Poulsen, TM Davis - Genome, 2016 - cdnsciencepub.com
As the product of interspecific hybridization between its two ancestral octoploid (2 n= 8 x=
56) species (Fragaria chiloensis and F. virginiana), the cultivated strawberry (F.× ananassa) …

Fluorescent In Situ Hybridization (FISH) on pachytene chromosomes as a tool for genome characterization

R Geurts, H de Jong - Legume Genomics: Methods and Protocols, 2013 - Springer
A growing number of international genome consortia have initiated large-scale sequencing
projects for most of the major crop species. This huge amount of information not only …