It has been a controversial issue as to how many DNA cytosine methyltransferase
mammalian cells have and whether de novo methylation and maintenance methylation …

Previous attempts to express functional DNA cytosine methyltransferase (EC 2.1. 1.37) in
cells transfected with the available Dnmt cDNAs have met with little or no success. We show …

Gene targeting in embryonic stem (ES) cells has been used to mutate the murine DNA
methyltransferase gene. ES cell lines homozygous for the mutation were generated by …

The mechanisms that establish and maintain methylation patterns in the mammalian
genome are very poorly understood, even though perturbations of methylation patterns lead …

We have shown previously that de novo methylation activities persist in mouse embryonic
stem (ES) cells homozygous for a null mutation of Dnmt1 that encodes the major DNA …

Trace levels of 5-methylcytosine persist in the DNA of mouse embryonic stem cells that are
homozygous for null mutations in Dnmt1, the gene for the one previously recognized …

The mouse genome experiences a large decrease in net 5‐methylcytosine between
fertilization and implantation; de novo methylation brings 5‐methylcytosine to adult somatic …

Methyl-accepting assays and a sensitive method for labeling specific CpG sites have been
used to show that the DNA of F9 embryonal carcinoma cells decreases in 5-methylcytosine …

DNA (cytosine-5)-methyltransferases (EC 2.1. 1.37) maintain patterns of methylated cytosine
residues in the mammalian genome; faithful maintenance of methylation patterns is required …

The steady state level of DNA methyltransferase mRNA is markedly increased as growth-
arrested Balb/c 3T3 cells progress into the S phase of the cell cycle. mRNA abundance is …