Using biosensor technology, it is possible to measure protein concentration when the
binding of the protein to an appropriate ligand immobilized on the sensor surface is totally …

The theory of a method for determining active concentration of nonpurified protein samples
using the BIAcore biosensor technology has been developed. The method relies on change …

Whereas the concentration of a biomolecule simply refers to the amount of chemical
substance per unit of volume, its active concentration refers to a relational parameter that …

Accurate determination of kinetic rate constants for interacting biomolecules requires
knowledge of the active concentrations of the participating molecules. Also, in other …

I examined the penetration of the hydrodynamic flow into a polymer matrix immobilized by
grafting to a surface, such as used in optical biosensors designed to measure binding …

Protein concentration data are required for understanding protein interactions and are a
prerequisite for the determination of affinity and kinetic properties. It is vital for the judgment …

Many techniques rely on the binding activity of surface-immobilized proteins, including
antibody-based affinity biosensors for the detection of analytes, immunoassays, protein …

A computer-controlled immunological binding assay intended for monitoring concentrations
of interesting metabolites during bioprocesses has been developed. The main focus has …

To explore the variability in biosensor studies, 150 participants from 20 countries were given
the same protein samples and asked to determine kinetic rate constants for the interaction …

The use of a simple device by which a layer of solvent may be deposited onto a solution of
an optically absorbing solute in a cylindrical quartz tube, without substantial mixing of …