Lamb birth weight is affected by culture system utilized during in vitro pre-elongation development of ovine embryos

JG Thompson, DK Gardner, P Anne Pugh… - Biology of …, 1995 - academic.oup.com
It has previously been reported that ovine embryos cultured in Synthetic Oviduct Fluid
medium supplemented with 20% human serum (SOF+ HS) develop into lambs with a high …

Development of ovine embryos in synthetic oviductal fluid containing amino acids at oviductal fluid concentrations

SK Walker, JL Hill, DO Kleemann… - Biology of …, 1996 - academic.oup.com
The effects of supplementing synthetic oviductal fluid (SOF) with amino acids, at oviductal
fluid concentrations, on the development of ovine in vitro-matured/in vitro-fertilized embryos …

Aberrant fetal growth and development after in vitro culture of sheep zygotes

KD Sinclair, TG McEvoy, EK Maxfield… - …, 1999 - rep.bioscientifica.com
The effects of in vitro culture systems for sheep zygotes on subsequent fetal growth and
development to day 61 and day 125 of gestation were studied. Zygotes recovered from …

Embryo viability, duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo

P Holm, SK Walker, RF Seamark - Reproduction, 1996 - rep.bioscientifica.com
The influence of various in vitro procedures on embryo survival and the production of normal
offspring was investigated in sheep. Zygotes produced from in vitro matured (IVM) and …

Comparison between in vivo-derived and in vitro-produced pre-elongation embryos from domestic ruminants

JG Thompson - Reproduction, Fertility and Development, 1997 - CSIRO Publishing
In vitro production of ruminant embryos has become routine and is increasingly available as
a commercial service to dairy, meat and wool producers. However, the efficiency of …

Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: amino acids …

DK Gardner, M Lane, A Spitzer… - Biology of reproduction, 1994 - academic.oup.com
The aim of this study was to develop a serum-free culture system that could support high
levels of cleavage and blastocyst formation from sheep zygotes developed in vitro. To this …

Successful culture in vitro of sheep and cattle ova

HR Tervit, DG Whittingham, LEA Rowson - Reproduction, 1972 - rep.bioscientifica.com
Fertilized sheep and cattle ova have not been reported to develop readily during culture in
vitro. Up to 60% of sheep morulae develop normally during culture (Moor & Cragle, 1971) …

Culture of one-and two-cell bovine embryos to the blastocyst stage in the ovine oviduct

WH Eyestone, ML Leibfried-Rutledge, DL Northey… - Theriogenology, 1987 - Elsevier
The ovine oviduct was evaluated as a culture system for early bovine embryos. One-to two-
cell embryos were collected from superovulated heifers killed 36 or 48 h after the onset of …

In vitro culture of sheep embryos without co-culture: successes and perspectives

SK Walker, TM Heard, RF Seamark - Theriogenology, 1992 - Elsevier
The finding that zygotes (one-cell embryos) from several livestock species can be routinely
cultured in relatively simple media to the blastocyst stage questions the nature of the …

Co-culture of ovine ova with oviductal cells in medium 199

CE Rexroad Jr, AM Powell - Journal of animal science, 1988 - academic.oup.com
Three experiments were conducted to test the suitability of medium 199 supplemented with
10% fetal calf serum (M199FCS) as a medium for co-culture of one-cell sheep ova with …