… Method: We estimated the errors of sample withdrawal and extraction, reverse transcription
(RT), and qPCR that are introduced into measurements of mRNA concentrations. We …

… of the two targets under optimized conditions that included a 5 min pre‐incubation with both
sets of primers at ambient temperature, followed by reverse transcription at 60C for 3 min. All …

… and optimize the one step reverse transcription quantitative … Primers were designed and
experimentally optimized to be … one-step RT-qPCR design was optimized for both miRNA targets …

… The type of primers used in the reverse transcription … for the reverse transcription reaction
overcomes the variability in yield, whereas treatment with DNase I prior to reverse transcription …

… The reverse transcription (RT) 4 of RNA to cDNA is an essential first step for many
research … Those that have investigated this matter with regard to reverse transcription real-time …

… One microgram total RNA was reverse transcribed using the Superscript III Reverse
Transcriptase (Invitrogen) with 250 ng random hexamer primers (Pharmacia, Uppsala, Sweden) …

… Reverse transcription coupled to the polymerase chain reaction (RT–PCR) is commonly …
as a template during reverse transcription, in which the enzyme reverse transcriptase (RT) …

… Using a single-tube assay and operating at higher reverse transcription temperature, we …
per tube for reverse transcription. This is, in part, also because of our use of an optimized RNA …

… and probes were designed for a TaqMan ® -based real-time reverse transcription-polymerase
chain reaction (RT-PCR) for norovirus detection purposes. The assay was optimized and …

… We present the clinical validation of a pH-dependent colorimetric reverse transcription
loop-mediated isothermal amplification (RT-LAMP) for SARS-CoV-2 detection. The method …