In 1925, 5-methylcytosine was first reported in bacteria. However, its biological importance
was not intuitive for several decades. After this initial lag, the ubiquitous presence of this …

Cells differentiate and progress through development guided by a dynamic chromatin
landscape that mediates gene expression programmes. During development, mammalian …

Single-cell RNA sequencing has revealed extensive transcriptional cell state diversity in
cancer, often observed independently of genetic heterogeneity, raising the central question …

Cell type-specific gene expression relies on transcription factors (TFs) binding DNA
sequence motifs embedded in chromatin. Understanding how motifs are accessed in …

Characterizing genetic influences on DNA methylation (DNAm) provides an opportunity to
understand mechanisms underpinning gene regulation and disease. In the present study …

Polycystic ovary syndrome (PCOS) is the main cause of female infertility worldwide and is
associated with a substantially increased lifetime risk of comorbidities, including type 2 …

DNA methylation (DNAme) and histone post-translational modifications (PTMs) have
important roles in transcriptional regulation. Although many reports have characterized the …

DNA methylation is a key layer of epigenetic regulation. The deposition of methylation marks
relies on the catalytic activity of DNA methyltransferases (DNMTs), and their active removal …

Chromatin accessibility is a hallmark of regulatory regions, entails transcription factor (TF)
binding and requires nucleosomal reorganization. However, it remains unclear how …

Background Cytosine DNA methylation is widely described as a transcriptional repressive
mark with the capacity to silence promoters. Epigenome engineering techniques enable …