Since the completion of the human genome project in 2003, extraordinary progress has
been made in genome sequencing technologies, which has led to a decreased cost per …

In the burgeoning field of epigenetics, there are several methods available to determine the
methylation status of DNA samples. However, choosing the method that is best suited to …

Repetitive DNA sequences are abundant in a broad range of species, from bacteria to
mammals, and they cover nearly half of the human genome. Repeats have always …

Polycomb group proteins maintain the gene-expression pattern of different cells that is set
during early development by regulating chromatin structure. In mammals, two main …

DNA cytosine methylation is a central epigenetic modification that has essential roles in
cellular processes including genome regulation, development and disease. Here we …

Methylation of cytosine bases in DNA provides a layer of epigenetic control in many
eukaryotes that has important implications for normal biology and disease. Therefore …

Genome-wide mapping of 5-methylcytosine is of broad interest to many fields of biology and
medicine. A variety of methods have been developed, and several have recently been …

Emerging single-cell epigenomic methods are being developed with the exciting potential to
transform our knowledge of gene regulation. Here we review available techniques and …

Human embryonic stem cells (hESCs) share an identical genome with lineage-committed
cells, yet possess the remarkable properties of self-renewal and pluripotency. The diverse …

Genome-wide dynamic changes in DNA methylation are indispensable for germline
development and genomic imprinting in mammals. Here, we report single-base resolution …