An updated structural classification of replicative DNA polymerases
Replicative DNA polymerases are nano-machines essential to life, which have evolved the
ability to copy the genome with high fidelity and high processivity. In contrast with cellular …
ability to copy the genome with high fidelity and high processivity. In contrast with cellular …
Evolution of replicative DNA polymerases in archaea and their contributions to the eukaryotic replication machinery
The elaborate eukaryotic DNA replication machinery evolved from the archaeal ancestors
that themselves show considerable complexity. Here we discuss the comparative genomic …
that themselves show considerable complexity. Here we discuss the comparative genomic …
Archaeal DNA replication
DNA replication is essential for all life forms. Although the process is fundamentally
conserved in the three domains of life, bioinformatic, biochemical, structural, and genetic …
conserved in the three domains of life, bioinformatic, biochemical, structural, and genetic …
Shared active site architecture between archaeal PolD and multi-subunit RNA polymerases revealed by X-ray crystallography
Archaeal replicative DNA polymerase D (PolD) constitute an atypical class of DNA
polymerases made of a proofreading exonuclease subunit (DP1) and a larger polymerase …
polymerases made of a proofreading exonuclease subunit (DP1) and a larger polymerase …
Family D DNA polymerase interacts with GINS to promote CMG-helicase in the archaeal replisome
K Oki, M Nagata, T Yamagami, T Numata… - Nucleic acids …, 2022 - academic.oup.com
Genomic DNA replication requires replisome assembly. We show here the molecular
mechanism by which CMG (GAN–MCM–GINS)-like helicase cooperates with the family D …
mechanism by which CMG (GAN–MCM–GINS)-like helicase cooperates with the family D …
Characterization of Family D DNA polymerase from Thermococcus sp. 9°N
L Greenough, JF Menin, NS Desai, Z Kelman… - Extremophiles, 2014 - Springer
Accurate DNA replication is essential for maintenance of every genome. All archaeal
genomes except Crenarchaea, encode for a member of Family B (polB) and Family D (polD) …
genomes except Crenarchaea, encode for a member of Family B (polB) and Family D (polD) …
Novel inhibition of archaeal family-D DNA polymerase by uracil
TT Richardson, L Gilroy, Y Ishino… - Nucleic Acids …, 2013 - academic.oup.com
Archaeal family-D DNA polymerase is inhibited by the presence of uracil in DNA template
strands. When the enzyme encounters uracil, following three parameters change: DNA …
strands. When the enzyme encounters uracil, following three parameters change: DNA …
PCR performance of a thermostable heterodimeric archaeal DNA polymerase
T Killelea, C Ralec, A Bossé, G Henneke - Frontiers in microbiology, 2014 - frontiersin.org
DNA polymerases are versatile tools used in numerous important molecular biological core
technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome …
technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome …
Elucidating functions of DP1 and DP2 subunits from the Thermococcus kodakarensis family D DNA polymerase
N Takashima, S Ishino, K Oki, M Takafuji, T Yamagami… - Extremophiles, 2019 - Springer
DNA polymerase D (PolD), originally discovered in Pyrococcus furiosus, has no sequence
homology with any other DNA polymerase family. Genes encoding PolD are found in most of …
homology with any other DNA polymerase family. Genes encoding PolD are found in most of …
[HTML][HTML] Novel structure of an N-terminal domain that is crucial for the dimeric assembly and DNA-binding of an archaeal DNA polymerase D large subunit from …
I Matsui, Y Urushibata, Y Shen, E Matsui, H Yokoyama - FEBS letters, 2011 - Elsevier
Archaea-specific D-family DNA polymerase forms a heterotetramer consisting of two large
polymerase subunits and two small exonuclease subunits. The N-terminal (1–300) domain …
polymerase subunits and two small exonuclease subunits. The N-terminal (1–300) domain …