The nucleus of a differentiated cell can be reprogrammed to a totipotent state by exposure to
the cytoplasm of an enucleated oocyte, and the reconstructed nuclear transfer embryo can …

Somatic cell nuclear transfer (SCNT) offers the possibility of preserving endangered species.
It is one of the few technologies that avoids the loss of genetic variation and provides the …

Faulty epigenetic reprogramming of somatic nuclei is likely to be a major cause of low
success observed in all mammals produced through somatic cell nuclear transfer (SCNT). It …

Stem cells are characterized by their capability to self-renew and terminally differentiate into
multiple cell types. Somatic or adult stem cells have a finite self-renewal capacity and are …

Abnormal epigenetic modification is supposed to be one of factors accounting for inefficient
reprogramming of the donor cell nuclei in ooplasm after somatic cell nuclear transfer …

Gene expression changes during cell differentiation are thought to be coordinated by
histone modifications, but still little is known about the role of specific histone deacetylases …

Our group and others have found that the treatment of embryos with trichostatin A (TSA) after
cloning by somatic cell nuclear transfer (SCNT) results in a significant improvement in …

In the present study, we used the sand cat (Felis margarita) as a somatic cell donor to
evaluate whether cryopreservation of donor cells alters viability and epigenetic events in …

We previously found that treatment of both donor cells and early cloned embryos with
combination of 5-aza-2′-deoxycytidine (5-aza-dC) and trichostatin A (TSA) significantly …

Successful cloning by somatic cell nuclear transfer (SCNT) requires a reprogramming
process in which the epigenetic state of a differentiated donor nucleus must be converted …