Strategies to improve the efficiency of somatic cell nuclear transfer
Mammalian oocytes can reprogram differentiated somatic cells into a totipotent state through
somatic cell nuclear transfer (SCNT), which is known as cloning. Although many mammalian …
somatic cell nuclear transfer (SCNT), which is known as cloning. Although many mammalian …
25th Anniversary of cloning by somatic-cell nuclear transfer: Scientific and technological approaches to improve SCNT efficiency in farm animals and pets
P Loi, L Palazzese, PA Scapolo, J Fulka… - …, 2021 - rep.bioscientifica.com
The birth of Dolly through somatic cell nuclear transfer (SCNT) was a major scientific
breakthrough of the last century. Yet, while significant progress has been achieved across …
breakthrough of the last century. Yet, while significant progress has been achieved across …
Oviduct epithelial cells‐derived extracellular vesicles improve preimplantation developmental competence of in vitro produced porcine parthenogenetic and cloned …
X Fang, BM Tanga, S Bang, G Seong… - Molecular …, 2022 - Wiley Online Library
Extracellular vesicles (EVs) carry bioactive cargoes involved in the early preimplantation
development. This study investigated the effects of EVs obtained from an oviductal epithelial …
development. This study investigated the effects of EVs obtained from an oviductal epithelial …
Vitamin C enhances porcine cloned embryo development and improves the derivation of embryonic stem-like cells
X Fang, BM Tanga, S Bang, G Seong, IM Saadeldin… - Reproductive …, 2022 - Elsevier
abstract Porcine cloning through somatic cell nuclear transfer (SCNT) has been widely used
in biotechnology for generating animal disease models and genetically modified animals for …
in biotechnology for generating animal disease models and genetically modified animals for …
[HTML][HTML] Combined chaetocin/trichostatin a treatment improves the epigenetic modification and developmental competence of porcine somatic cell nuclear transfer …
PS Jeong, HJ Yang, SH Park, MA Gwon… - Frontiers in Cell and …, 2021 - frontiersin.org
Developmental defects in somatic cell nuclear transfer (SCNT) embryos are principally
attributable to incomplete epigenetic reprogramming. Small-molecule inhibitors such as …
attributable to incomplete epigenetic reprogramming. Small-molecule inhibitors such as …
Whole-genome methylation analysis reveals epigenetic variation between wild-type and nontransgenic cloned, ASMT transgenic cloned dairy goats generated by the …
H Wu, W Zhou, H Liu, X Cui, W Ma, H Wu, G Li… - Journal of Animal …, 2022 - Springer
Background SCNT (somatic cell nuclear transfer) is of great significance to biological
research and also to the livestock breeding. However, the survival rate of the SCNT cloned …
research and also to the livestock breeding. However, the survival rate of the SCNT cloned …
Conservation biology and reproduction in a time of developmental plasticity
WV Holt, P Comizzoli - Biomolecules, 2022 - mdpi.com
The objective of this review is to ask whether, and how, principles in conservation biology
may need to be revisited in light of new knowledge about the power of epigenetics to alter …
may need to be revisited in light of new knowledge about the power of epigenetics to alter …
A new toolbox in experimental embryology—Alternative model organisms for studying preimplantation development
C Springer, E Wolf, K Simmet - Journal of Developmental Biology, 2021 - mdpi.com
Preimplantation development is well conserved across mammalian species, but major
differences in developmental kinetics, regulation of early lineage differentiation and …
differences in developmental kinetics, regulation of early lineage differentiation and …
Transgenerational Epigenetic DNA Methylation Editing and Human Disease
JD Tompkins - Biomolecules, 2023 - mdpi.com
During gestation, maternal (F0), embryonic (F1), and migrating primordial germ cell (F2)
genomes can be simultaneously exposed to environmental influences. Accumulating …
genomes can be simultaneously exposed to environmental influences. Accumulating …
Suppression of endogenous retroviral enhancers in mouse embryos derived from somatic cell nuclear transfer
Endogenous retroviruses (ERVs) in the mammalian genome play diverse roles in embryonic
development. These developmentally related ERVs are generally repressed in somatic cells …
development. These developmentally related ERVs are generally repressed in somatic cells …