Dynamic binding of transcription factors (TFs) to regulatory elements controls transcriptional
states throughout organism development. Epigenetics modifications, such as DNA …

The character of a cell is defined by its constituent proteins, which are the result of specific
patterns of gene expression. Crucial determinants of gene expression patterns are DNA …

A general approach for heritably altering gene expression has the potential to enable many
discovery and therapeutic efforts. Here, we present CRISPRoff—a programmable epigenetic …

Background Cytosine DNA methylation is widely described as a transcriptional repressive
mark with the capacity to silence promoters. Epigenome engineering techniques enable …

To gain insight into the function of DNA methylation at cis-regulatory regions and its impact
on gene expression, we measured methylation, RNA polymerase occupancy and histone …

We examined the abundance of microsatellites with repeated unit lengths of 1–6 base pairs
in several eukaryotic taxonomic groups: primates, rodents, other mammals, nonmammalian …

Methylation of mammalian DNA can lead to repression of transcription and alteration of
chromatin structure. Recent evidence suggests that both effects are the result of an …

In most higher organisms, DNA is modified after synthesis by the enzymatic conversion of
many cytosine residues to 5-methylcytosine. For several years, control of gene activity by …

We describe the construction, evaluation and application of cDNA libraries from unfertilized
oocytes, and single 2-cell, 4-cell, 7-cell and blastocyst stage embryos. The corresponding …

Phenotypic variation is traditionally parsed into components that are directed by genetic and
environmental variation. The line between these two components is blurred by inherited …