Applications of CRISPR–Cas in agriculture and plant biotechnology

H Zhu, C Li, C Gao - Nature Reviews Molecular Cell Biology, 2020 - nature.com
The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …

Epigenome plasticity in plants

JPB Lloyd, R Lister - Nature Reviews Genetics, 2022 - nature.com
Plant intra-individual and inter-individual variation can be determined by the epigenome, a
set of covalent modifications of DNA and chromatin that can alter genome structure and …

RNA-directed DNA methylation

RM Erdmann, CL Picard - PLoS genetics, 2020 - journals.plos.org
RNA-directed DNA methylation (RdDM) is a biological process in which non-coding RNA
molecules direct the addition of DNA methylation to specific DNA sequences. The RdDM …

The emerging and uncultivated potential of CRISPR technology in plant science

Y Zhang, AA Malzahn, S Sretenovic, Y Qi - Nature Plants, 2019 - nature.com
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

Designing future crops: challenges and strategies for sustainable agriculture

Z Tian, JW Wang, J Li, B Han - The Plant Journal, 2021 - Wiley Online Library
Crop production is facing unprecedented challenges. Despite the fact that the food supply
has significantly increased over the past half‐century,~ 8.9 and 14.3% people are still …

CRISPR–Act3. 0 for highly efficient multiplexed gene activation in plants

C Pan, X Wu, K Markel, AA Malzahn, N Kundagrami… - Nature Plants, 2021 - nature.com
RNA-guided CRISPR activation (CRISPRa) systems have been developed in plants.
However, the simultaneous activation of multiple genes remains challenging. Here, we …

Fast-forward breeding for a food-secure world

RK Varshney, A Bohra, M Roorkiwal, R Barmukh… - Trends in Genetics, 2021 - cell.com
Crop production systems need to expand their outputs sustainably to feed a burgeoning
human population. Advances in genome sequencing technologies combined with efficient …

[HTML][HTML] CRISPR/Cas: A powerful tool for gene function study and crop improvement

D Zhang, Z Zhang, T Unver, B Zhang - Journal of Advanced Research, 2021 - Elsevier
Background It is a long-standing goal of scientists and breeders to precisely control a gene
for studying its function as well as improving crop yield, quality, and tolerance to various …

Principles, applications, and biosafety of plant genome editing using CRISPR-Cas9

K El-Mounadi, ML Morales-Floriano… - Frontiers in plant …, 2020 - frontiersin.org
The terms genome engineering, genome editing, and gene editing, refer to modifications
(insertions, deletions, substitutions) in the genome of a living organism. The most widely …

Natural variation in crops: realized understanding, continuing promise

Y Liang, HJ Liu, J Yan, F Tian - Annual review of plant biology, 2021 - annualreviews.org
Crops feed the world's population and shape human civilization. The improvement of crop
productivity has been ongoing for almost 10,000 years and has evolved from an experience …