Feeding the ever-growing population is a major challenge, especially in light of rapidly
changing climate conditions. Genome editing is set to revolutionize plant breeding and …

The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …

CRISPR–Cas genetic engineering of plants holds tremendous potential for providing food
security, battling biotic and abiotic crop stresses caused by climate change, and for …

The rapid development of the CRISPR–Cas9,–Cas12a and–Cas12b genome editing
systems has greatly fuelled basic and translational plant research,,,,–. DNA targeting by …

RNA-guided CRISPR activation (CRISPRa) systems have been developed in plants.
However, the simultaneous activation of multiple genes remains challenging. Here, we …

CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided
genome engineering in plants. However, these systems are mostly used separately, leaving …

CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …

The terms genome engineering, genome editing, and gene editing, refer to modifications
(insertions, deletions, substitutions) in the genome of a living organism. The most widely …

CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

Ensuring food security for an ever-growing global population while adapting to climate
change is the main challenge for agriculture in the 21st century. Although new technologies …