DNA polymerases as useful reagents for biotechnology–the history of developmental research in the field

S Ishino, Y Ishino - Frontiers in microbiology, 2014 - frontiersin.org
DNA polymerase is a ubiquitous enzyme that synthesizes complementary DNA strands
according to the template DNA in living cells. Multiple enzymes have been identified from …

Archaeal DNA replication: identifying the pieces to solve a puzzle

IKO Cann, Y Ishino - Genetics, 1999 - academic.oup.com
Archaeal organisms are currently recognized as very exciting and useful experimental
materials. A major challenge to molecular biologists studying the biology of Archaea is their …

Complete Genome Sequence of the Methanogenic Archaeon, Methanococcus jannaschii

CJ Bult, O White, GJ Olsen, L Zhou, RD Fleischmann… - Science, 1996 - science.org
The complete 1.66-megabase pair genome sequence of an autotrophic archaeon,
Methanococcus jannaschii, and its 58-and 16-kilobase pair extrachromosomal elements …

A hypothesis for DNA viruses as the origin of eukaryotic replication proteins

LP Villarreal, VR DeFilippis - Journal of virology, 2000 - Am Soc Microbiol
The eukaryotic replicative DNA polymerases are similar to those of large DNA viruses of
eukaryotic and bacterial T4 phages but not to those of eubacteria. We develop and examine …

Thermostable DNA polymerases

FB Perler, S Kumar, H Kong - Advances in protein chemistry, 1996 - Elsevier
Publisher Summary This chapter describes DNA polymerases of mesophiles and covers
DNA polymerases of thermophilic Eubacteria and Archaea. It covers both thermophiles …

Structural framework for the mechanism of archaeal exosomes in RNA processing

K Büttner, K Wenig, KP Hopfner - Molecular cell, 2005 - cell.com
Exosomes emerge as central 3′→ 5′ RNA processing and degradation machineries in
eukaryotes and archaea. We determined crystal structures of two 230 kDa nine subunit …

A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus: gene cloning, expression, and characterization

T Uemori, Y Sato, I Kato, H Doi, Y Ishino - Genes to Cells, 1997 - Wiley Online Library
Background: In many respects Archaea are much more like eukaryotes than prokaryotes
with respect to the conservation of many of the components involved in transcription …

A heterodimeric DNA polymerase: evidence that members of Euryarchaeota possess a distinct DNA polymerase

IKO Cann, K Komori, H Toh… - Proceedings of the …, 1998 - National Acad Sciences
We describe here a DNA polymerase family highly conserved in Euryarchaeota, a
subdomain of Archaea. The DNA polymerase is composed of two proteins, DP1 and DP2 …

Overview of thermostable DNA polymerases for classical PCR applications: from molecular and biochemical fundamentals to commercial systems

K Terpe - Applied microbiology and biotechnology, 2013 - Springer
During the genomics era, the use of thermostable DNA polymerases increased greatly.
Many were identified and described—mainly of the genera Thermus, Thermococcus and …

A Novel DNA Polymerase Family Found inArchaea

Y Ishino, K Komori, IKO Cann, Y Koga - Journal of bacteriology, 1998 - Am Soc Microbiol
One of the most puzzling results from the complete genome sequence of the methanogenic
archaeon Methanococcus jannaschii was that the organism may have only one DNA …