Isothermal nucleic acid amplification techniques are used to detect a variety of molecules
such as RNAs, DNAs and proteins. When compared to the gold standard polymerase chain …

The steady increase in the number of newly isolated extremophilic microorganisms and the
discovery of their enzymes by academic and industrial institutions underlines the enormous …

A simple isothermal nucleic-acid amplification reaction, primer generation–rolling circle
amplification (PG–RCA), was developed to detect specific nucleic-acid sequences of sample …

Rapid isothermal nucleic acid amplification technologies can enable diagnosis of human
pathogens and genetic variations in a simple, inexpensive, user-friendly format. The …

DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a
template. They are essential for nucleic acid metabolism and for DNA replication and repair …

Thermostable DNA polymerases have a crucial role in current methods of DNA amplification
and sequencing. Major improvements in these methods have been made in recent years …

Isothermal nucleic acid amplification is becoming increasingly important for molecular
diagnostics. Therefore, new computational tools are needed to facilitate assay design. In the …

DNA polymerases (pols) catalyse the synthesis of DNA. This reaction requires a primer-
template DNA in order to grow from the 3′ OH end of the primer along the template. On the …

Highly efficient DNA synthesis without template and primer DNAs occurs when N. BspD6I
DNA nickase is added to a reaction mixture containing deoxynucleoside triphosphates and …

DNA methylation is a predominant epigenetic modification that plays crucial roles in various
cellular processes. DNA methyltransferase (MTase) is responsible for DNA methylation, and …