[HTML][HTML] A review of reaction enhancement strategies for isothermal nucleic acid amplification reactions
B Özay, SE McCalla - Sensors and Actuators Reports, 2021 - Elsevier
Isothermal nucleic acid amplification techniques are used to detect a variety of molecules
such as RNAs, DNAs and proteins. When compared to the gold standard polymerase chain …
such as RNAs, DNAs and proteins. When compared to the gold standard polymerase chain …
Extreme environments as a resource for microorganisms and novel biocatalysts
G Antranikian, CE Vorgias, C Bertoldo - Marine biotechnology I, 2005 - Springer
The steady increase in the number of newly isolated extremophilic microorganisms and the
discovery of their enzymes by academic and industrial institutions underlines the enormous …
discovery of their enzymes by academic and industrial institutions underlines the enormous …
Sensitive isothermal detection of nucleic-acid sequence by primer generation–rolling circle amplification
T Murakami, J Sumaoka, M Komiyama - Nucleic acids research, 2009 - academic.oup.com
A simple isothermal nucleic-acid amplification reaction, primer generation–rolling circle
amplification (PG–RCA), was developed to detect specific nucleic-acid sequences of sample …
amplification (PG–RCA), was developed to detect specific nucleic-acid sequences of sample …
Specific versus nonspecific isothermal DNA amplification through thermophilic polymerase and nicking enzyme activities
E Tan, B Erwin, S Dames, T Ferguson, M Buechel… - Biochemistry, 2008 - ACS Publications
Rapid isothermal nucleic acid amplification technologies can enable diagnosis of human
pathogens and genetic variations in a simple, inexpensive, user-friendly format. The …
pathogens and genetic variations in a simple, inexpensive, user-friendly format. The …
[HTML][HTML] Bst polymerase—a humble relative of Taq polymerase
I Oscorbin, M Filipenko - Computational and Structural Biotechnology …, 2023 - Elsevier
DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a
template. They are essential for nucleic acid metabolism and for DNA replication and repair …
template. They are essential for nucleic acid metabolism and for DNA replication and repair …
Recent developments in the optimization of thermostable DNA polymerases for efficient applications
AR Pavlov, NV Pavlova, SA Kozyavkin… - Trends in …, 2004 - cell.com
Thermostable DNA polymerases have a crucial role in current methods of DNA amplification
and sequencing. Major improvements in these methods have been made in recent years …
and sequencing. Major improvements in these methods have been made in recent years …
Sequence dependence of isothermal DNA amplification via EXPAR
J Qian, TM Ferguson, DN Shinde… - Nucleic acids …, 2012 - academic.oup.com
Isothermal nucleic acid amplification is becoming increasingly important for molecular
diagnostics. Therefore, new computational tools are needed to facilitate assay design. In the …
diagnostics. Therefore, new computational tools are needed to facilitate assay design. In the …
De novo DNA synthesis by human DNA polymerase λ, DNA polymerase μ and terminal deoxyribonucleotidyl transferase
K Ramadan, IV Shevelev, G Maga… - Journal of molecular …, 2004 - Elsevier
DNA polymerases (pols) catalyse the synthesis of DNA. This reaction requires a primer-
template DNA in order to grow from the 3′ OH end of the primer along the template. On the …
template DNA in order to grow from the 3′ OH end of the primer along the template. On the …
N. BspD6I DNA nickase strongly stimulates template-independent synthesis of non-palindromic repetitive DNA by Bst DNA polymerase
NV Zyrina, LA Zheleznaya, EV Dvoretsky, VD Vasiliev… - 2007 - degruyter.com
Highly efficient DNA synthesis without template and primer DNAs occurs when N. BspD6I
DNA nickase is added to a reaction mixture containing deoxynucleoside triphosphates and …
DNA nickase is added to a reaction mixture containing deoxynucleoside triphosphates and …
[HTML][HTML] Single-ribonucleotide repair-mediated ligation-dependent cycling signal amplification for sensitive and specific detection of DNA methyltransferase
L Wang, X Han, C Li, C Zhang - Chemical science, 2018 - pubs.rsc.org
DNA methylation is a predominant epigenetic modification that plays crucial roles in various
cellular processes. DNA methyltransferase (MTase) is responsible for DNA methylation, and …
cellular processes. DNA methyltransferase (MTase) is responsible for DNA methylation, and …