Structural study of immunoaffinity-purified DNA polymerase α-DNA primase complex from calf thymus

K Tamai, K Kojima, T Hanaichi, S Masaki… - … et Biophysica Acta (BBA …, 1988 - Elsevier
The DNA polymerase α-DNA primase complex was purified over 17 000-fold to near
homogeneity from calf thymus using an immunoaffinity column. Sodium dodecyl sulfate gel …

E. coli DNA polymerase I as a reverse transcriptase.

M Ricchetti, H Buc - The EMBO journal, 1993 - embopress.org
The ability of Escherichia coli DNA polymerase I to retrotranscribe an RNA template was
examined under steady‐state conditions, using a primer extension assay which allows …

Intracellular localization and metabolism of DNA polymerase α in human cells visualized with monoclonal antibody

H Nakamura, T Morita, S Masaki, S Yoshida - Experimental cell research, 1984 - Elsevier
Indirect immunofluorescence microscopy with monoclonal antibody against DNA
polymerase α revealed the intranuclear localization of DNA polymerase α in G1, S, and G2 …

Inhibition of DNA polymerase α by gossypol

LJ Rosenberg, RC Adlakha, DM Desai… - Biochimica et Biophysica …, 1986 - Elsevier
Our earlier studies have shown that gossypol is a specific inhibitor of DNA synthesis in
cultured cells at low doses. In an attempt to determine the mechanism for the inhibition of …

Detection of enzymatic activities in sodium dodecyl sulfate-polyacrylamide gels: DNA polymerases as model enzymes

A Blank, JR Silber, MP Thelen, CA Dekker - Analytical biochemistry, 1983 - Elsevier
Recent techniques for detecting the catalytic activity of enzymes in sodium dodecyl sulfate
(SDS)-polyacrylamide gels have been hampered by lack of reproducibility associated with …

Utilization of 2′-Deoxy-6-thioguanosine 5′-Triphosphate in DNA Synthesis in vitro by DNA Polymerase α from Calf Thymus

S Yoshida, M Yamada, S Masaki, M Saneyoshi - Cancer research, 1979 - AACR
Abstract Chemically synthesized β-2′-deoxy-6-thioguanosine 5′-triphosphate, a putative
active form of β-2′-deoxy-6-thioguanosine, was used efficiently as a substrate for DNA …

Purification of a 9S DNA polymerase. alpha. species from calf thymus

F Grosse, G Krauss - Biochemistry, 1981 - ACS Publications
Frank Grosse and Gerhard Krauss* abstract: A DNA polymerase a species from calf thymus
has been purified 12000-fold to near homogeneity. The en-zyme sediments under high salt …

Eukaryotic DNA polymerases

M Fry - Enzymes of nucleic acid synthesis and modification, 2018 - taylorfrancis.com
This chapter focuses on cellular deoxyribonucleic acid (DNA) polymerases of higher
eukaryotes and only occasional note is made on enzymes of lower eukaryotes. It explores …

Terminal deoxynucleotidyltransferase of 60,000 daltons from mouse, rat, and calf thymus. Purification by immunoadsorbent chromatography and comparison of …

H Nakamura, K Tanabe, S Yoshida, T Morita - Journal of Biological …, 1981 - Elsevier
For rapid simple purification of terminal deoxynucleotidyltransferase from limited amounts of
tissues, we have developed an immunoadsorbent column chromatographic method using …

Terminal differentiation of human erythroleukemia cell line K562 induced by aphidicolin

T Murate, Y Kagami, T Hotta, T Yoshida, H Saito… - Experimental cell …, 1990 - Elsevier
To analyze the relationship between differentiation and DNA replication, the effect of
aphidicolin, a specific inhibitor for DNA polymerase α, was measured with respect to …