All cells employ architectural proteins to confine and organize their chromosomes, and to
prevent the otherwise thermodynamically favored collapse of concentrated DNA into …

Publisher Summary This chapter describes DNA polymerases of mesophiles and covers
DNA polymerases of thermophilic Eubacteria and Archaea. It covers both thermophiles …

Only in the last decade have microorganisms been discovered which grow near or above
100° C. The enzymes that are formed by these extremely thermophilic (growth temperature …

Hot springs ecosystem is the most ancient continuously inhabited ecosystem on earth which
harbors diverse thermophilic bacteria and archaea distributed worldwide. Life in extreme …

We report the crystal structure of an NH2-terminal 388-residue fragment of T4 DNA
polymerase (protein N388) refined at 2.2 Å resolution. This fragment contains both the 3 '− 5 …

Cenarchaeum symbiosum, an archaeon which lives in specific association with a marine
sponge, belongs to a recently recognized nonthermophilic crenarchaeotal group that …

Over the past three decades polyacrylamide gel electrophoresis in the presence of sodium
dodecyl sulfate (SDS–PAGE) 2 has emerged as a method of choice for the analysis of the …

Thermophilic and hyperthermophilic microorganisms are found as normal inhabitants of
continental and submarine volcanic areas, geothermally heated sea-sediments and …

To minimize the large number of mispairs during genome duplication owing to the large
amount of DNA to be synthesized, many replicative polymerases have accessory domains …

ABSTRACT DNA polymerase activities in fractionated cell extract of Aeropyrum pernix, a
hyperthermophilic crenarchaeote, were investigated. Aphidicolin-sensitive (fraction I) and …