Developments in industrially important thermostable enzymes: a review
GD Haki, SK Rakshit - Bioresource technology, 2003 - Elsevier
Cellular components of thermophilic organisms (enzymes, proteins and nucleic acids) are
also thermostable. Apart from high temperature they are also known to withstand …
also thermostable. Apart from high temperature they are also known to withstand …
Extremophiles as a source of novel enzymes for industrial application
F Niehaus, C Bertoldo, M Kähler… - Applied microbiology and …, 1999 - Springer
Extremophilic microorganisms are adapted to survive in ecological niches such as at high
temperatures, extremes of pH, high salt concentrations and high pressure. These …
temperatures, extremes of pH, high salt concentrations and high pressure. These …
Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening
VL Makarov, Y Hirose, JP Langmore - Cell, 1997 - cell.com
The chromosomes of lower eukaryotes have short telomeric 3′ extensions. Using a primer-
extension/nick-translation technique and nondenaturing hybridization, we find long 3′ G …
extension/nick-translation technique and nondenaturing hybridization, we find long 3′ G …
High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5'to 3' …
FC Lawyer, S Stoffel, RK Saiki, SY Chang… - Genome …, 1993 - genome.cshlp.org
The Thermus aquaticus DNA polymerase I (Taq Pol I) gene was cloned into a plasmid
expression vector that utilizes the strong bacteriophage lambda PL promoter. A truncated …
expression vector that utilizes the strong bacteriophage lambda PL promoter. A truncated …
Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase
TW Myers, DH Gelfand - Biochemistry, 1991 - ACS Publications
PCR Division, Cetus Corporation, Emeryville, California 94608 Received May 14, 1991;
Revised Manuscript Received June 11, 1991 abstract: A recombinant DNA polymerase …
Revised Manuscript Received June 11, 1991 abstract: A recombinant DNA polymerase …
Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR
MM Huang, N Arnheim, MF Goodman - Nucleic acids research, 1992 - academic.oup.com
Thermus aquaticus (Taq) DNA polymerase was used to measure the extension efficiency for
all configurations of matched and mismatched base pairs at template-primer 3′-termini …
all configurations of matched and mismatched base pairs at template-primer 3′-termini …
Structure-specific endonucleolytic cleavage of nucleic acids by eubacterial DNA polymerases
V Lyamichev, MAD Brow, JE Dahlberg - Science, 1993 - science.org
Previously known 5′ exonucleases of several eubacterial DNA polymerases have now
been shown to be structure-specific endonucleases that cleave single-stranded DNA or …
been shown to be structure-specific endonucleases that cleave single-stranded DNA or …
Oligonucleotide inhibitors ofTaqDNA polymerase facilitate detection of low copy number targets by PCR
C Dang, SD Jayasena - Journal of molecular biology, 1996 - Elsevier
A random sequence library of single stranded DNA was screened to isolate sequences with
high affinity forThermus aquaticusDNA polymerase (Taqpol), a thermostable enzyme …
high affinity forThermus aquaticusDNA polymerase (Taqpol), a thermostable enzyme …
Thermostable Bst DNA polymerase I lacks a 3′→ 5′ proofreading exonuclease activity
JM Aliotta, JJ Pelletier, JL Ware, LS Moran… - Genetic analysis …, 1996 - Elsevier
A thermostable DNA polymerase, the Bst DNA polymerase I, from Bacillus
stearothermophilus N3468 was prepared to near-homogeneity. The dominant species of the …
stearothermophilus N3468 was prepared to near-homogeneity. The dominant species of the …
Thermostable DNA polymerases
FB Perler, S Kumar, H Kong - Advances in protein chemistry, 1996 - Elsevier
Publisher Summary This chapter describes DNA polymerases of mesophiles and covers
DNA polymerases of thermophilic Eubacteria and Archaea. It covers both thermophiles …
DNA polymerases of thermophilic Eubacteria and Archaea. It covers both thermophiles …