Natural products of pentacyclic triterpenoids: from discovery to heterologous biosynthesis

Y Li, J Wang, L Li, W Song, M Li, X Hua… - Natural Product …, 2023 - pubs.rsc.org
Covering: up to 2022 Pentacyclic triterpenoids are important natural bioactive substances
that are widely present in plants and fungi. They have significant medicinal efficacy, play an …

Living microecological hydrogels for wound healing

G Chen, F Wang, X Zhang, Y Shang, Y Zhao - Science Advances, 2023 - science.org
Chronic hard-to-heal wounds draw great attention worldwide, as their treatments are limited
by infections and hypoxia. Inspired by the natural oxygen production capacity of algae and …

Promoter engineering of natural product biosynthetic gene clusters in actinomycetes: Concepts and applications

CH Ji, HW Je, H Kim, HS Kang - Natural Product Reports, 2024 - pubs.rsc.org
Covering 2011 to 2022Low titers of natural products in laboratory culture or fermentation
conditions have been one of the challenging issues in natural products research. Many …

Tailored UPRE2 variants for dynamic gene regulation in yeast

C Xiao, X Liu, Y Pan, Y Li, L Qin… - Proceedings of the …, 2024 - National Acad Sciences
Genetic elements are foundational in synthetic biology serving as vital building blocks. They
enable programming host cells for efficient production of valuable chemicals and …

Efficient Protein Expression and Biosynthetic Gene Cluster Regulation in Bacillus subtilis Driven by a T7-BOOST System

Y Wu, Y Li, Y Zhang, Y Liu, J Li, G Du, X Lv… - ACS Synthetic …, 2023 - ACS Publications
Bacillus subtilis is a generally recognized as safe microorganism that is widely used for
protein expression and chemical production, but has a limited number of genetic regulatory …

Engineered multiple translation initiation sites: a novel tool to enhance protein production in Bacillus licheniformis and other industrially relevant bacteria

M Zhang, J Song, J Xiao, J Jin, CT Nomura… - Nucleic Acids …, 2022 - academic.oup.com
Gram-positive bacteria are a nascent platform for synthetic biology and metabolic
engineering that can provide new opportunities for the production of biomolecules …

Isolation and Optimization of a Broad-Spectrum Synthetic Antimicrobial Peptide, Ap920-WI, from Arthrobacter sp. H5 for the Biological Control of Plant Diseases

L Zhao, MS Islam, P Song, L Zhu, W Dong - International Journal of …, 2023 - mdpi.com
Antimicrobial peptides (AMPs) are naturally occurring molecules found in various organisms
that can help to defend against invading microorganisms and reduce the likelihood of drug …

Engineering Escherichia coli for cost-effective production of medium-chain fatty acids from soy whey using an optimized galactose-based autoinduction system

Z Wang, Y Dai, F Azi, Z Wang, W Xu, D Wang… - Bioresource …, 2024 - Elsevier
Medium-chain fatty acids (MCFAs) are essential chemical feedstocks. Microbial production
of MCFAs offers an attractive alternative to conventional methods, but the costly media and …

Boosting the Heterologous Expression of d-Allulose 3-Epimerase in Bacillus subtilis through Protein Engineering and Catabolite-Responsive Element Box …

Z Liu, Y Wang, S Liu, X Guo, T Zhao… - Journal of Agricultural …, 2022 - ACS Publications
As a natural sweetener with low calories and various physiological activities, d-allulose has
drawn worldwide attention. Currently, d-allulose 3-epimerase (DAEase) is mainly used to …

Development of a glycerol-inducible expression system for high-yield heterologous protein production in Bacillus subtilis

L Han, Q Chen, J Luo, W Cui, Z Zhou - Microbiology Spectrum, 2022 - Am Soc Microbiol
The development of efficient, low-cost, and robust expression systems is important for the
mass production of proteins and natural products in large amounts using cell factories …