We report on the successful delivery of the Cre recombinase enzyme in the neural cells of
mice in vivo by simple coinjection with peptides derived from HIV-TAT. Cre delivery activates …

Background Understanding and manipulating gene function in physiological conditions is a
major objective for both fundamental and applied research. In contrast to other experimental …

Cell penetrating peptides (CPPs) are short peptides that can pass through cell membranes.
CPPs can facilitate the cellular entry of proteins, macromolecules, nanoparticles and drugs …

Background Inactivating genes in vivo is an important technique for establishing their
function in the adult nervous system. Unfortunately, conventional knockout mice may suffer …

The protein transduction technique enables the direct delivery of biologically active material
into mammalian cells [for review see 1, 2]. For this one can make use of the translocating …

Conditional mutagenesis is a powerful tool to analyze gene functions in mammalian cells.
The site-specific recombinase Cre can be used to recombine loxP-modified alleles under …

Delivery of therapeutic proteins into tissues and across the blood-brain barrier is severely
limited by the size and biochemical properties of the proteins. Here it is shown that …

Background Protein transduction is safer than viral vector-mediated transduction for the
delivery of a therapeutic protein into a cell. Fusion proteins with an arginine-rich cell …

Gene deletion has been a valuable tool for unraveling the mysteries of molecular biology.
Early approaches included gene trapping and gene targetting to disrupt or delete a gene …

The Cre recombinase (Cre) from bacteriophage P1 is an important tool for genetic
engineering in mammalian cells. We constructed lentiviral vectors that efficiently deliver Cre …