Whole genome DNA methylation analysis based on high throughput sequencing technology
N Li, M Ye, Y Li, Z Yan, LM Butcher, J Sun, X Han… - Methods, 2010 - Elsevier
There are numerous approaches to decipher a whole genome DNA methylation profile
(“methylome”), each varying in cost, throughput and resolution. The gold standard of these …
(“methylome”), each varying in cost, throughput and resolution. The gold standard of these …
Targeted DNA methylation analysis by next-generation sequencing
DR Masser, DR Stanford, WM Freeman - JoVE (Journal of Visualized …, 2015 - jove.com
The role of epigenetic processes in the control of gene expression has been known for a
number of years. DNA methylation at cytosine residues is of particular interest for epigenetic …
number of years. DNA methylation at cytosine residues is of particular interest for epigenetic …
[HTML][HTML] Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling
Sequencing-based approaches have led to new insights about DNA methylation. While
many different techniques for genome-scale mapping of DNA methylation have been …
many different techniques for genome-scale mapping of DNA methylation have been …
[HTML][HTML] Evaluation of whole-genome DNA methylation sequencing library preparation protocols
J Morrison, JM Koeman, BK Johnson, KK Foy… - Epigenetics & …, 2021 - Springer
Background With rapidly dropping sequencing cost, the popularity of whole-genome DNA
methylation sequencing has been on the rise. Multiple library preparation protocols currently …
methylation sequencing has been on the rise. Multiple library preparation protocols currently …
DNA methylation profiling: comparison of genome-wide sequencing methods and the Infinium Human Methylation 450 Bead Chip
DL Walker, AV Bhagwate, S Baheti, RL Smalley… - …, 2015 - Taylor & Francis
Aims: To compare the performance of four sequence-based and one microarray methods for
DNA methylation profiling. Methods: DNA from two cell lines were profiled by reduced …
DNA methylation profiling. Methods: DNA from two cell lines were profiled by reduced …
Estimating absolute methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods
Recent advancements in sequencing-based DNA methylation profiling methods provide an
unprecedented opportunity to map complete DNA methylomes. These include whole …
unprecedented opportunity to map complete DNA methylomes. These include whole …
Comparison of EM-seq and PBAT methylome library methods for low-input DNA
Y Han, GY Zheleznyakova, Y Marincevic-Zuniga… - Epigenetics, 2022 - Taylor & Francis
ABSTRACT DNA methylation is the most studied epigenetic mark involved in regulation of
gene expression. For low input samples, a limited number of methods for quantifying DNA …
gene expression. For low input samples, a limited number of methods for quantifying DNA …
[HTML][HTML] Profiling genome-wide DNA methylation
DNA methylation is an epigenetic modification that plays an important role in regulating
gene expression and therefore a broad range of biological processes and diseases. DNA …
gene expression and therefore a broad range of biological processes and diseases. DNA …
Enzymatic methyl sequencing detects DNA methylation at single-base resolution from picograms of DNA
R Vaisvila, VKC Ponnaluri, Z Sun… - Genome …, 2021 - genome.cshlp.org
Bisulfite sequencing detects 5mC and 5hmC at single-base resolution. However, bisulfite
treatment damages DNA, which results in fragmentation, DNA loss, and biased sequencing …
treatment damages DNA, which results in fragmentation, DNA loss, and biased sequencing …
DNA methylome analysis using short bisulfite sequencing data
Bisulfite conversion of genomic DNA combined with next-generation sequencing (BS-seq) is
widely used to measure the methylation state of a whole genome, the methylome, at single …
widely used to measure the methylation state of a whole genome, the methylome, at single …